In recent years, antimicrobial‐resistant Pseudomonas aeruginosa strains have increased in the veterinary field. Therefore, phage therapy has received significant attention as an approach for overcoming antimicrobial resistance. In this context, we isolated and characterized four Pseudomonas bacteriophages. Phylogenetic analysis showed that the isolated phages are novel Myoviridae Pbunavirus PB1‐like phages with ØR12 belonging to a different clade compared with the other three. These phages had distinct lytic activity against 22 P. aeruginosa veterinary isolates. The phage cocktail composed from the PB1‐like phages clearly inhibited the occurrence of the phage‐resistant variant, suggesting that these phages could be useful in phage therapy.

中文翻译：

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铜绿假单胞菌兽医分离株对肺炎病毒PB1样噬菌体的敏感性。

近年来，在兽医领域中，对铜绿假单胞菌具有抗药性的菌株有所增加。因此，噬菌体疗法作为克服抗药性的一种方法受到了广泛的关注。在这种情况下，我们分离并鉴定了四种假单胞菌噬菌体。系统发育分析表明，分离的噬菌体是新型的Myoviridae Pbunavirus PB1样噬菌体，与其他三个相比，ØR12属于不同进化枝。这些噬菌体对22种铜绿假单胞菌具有独特的裂解活性兽医分离株。由PB1样噬菌体组成的噬菌体混合物明显抑制了噬菌体抗性变异体的发生，这表明这些噬菌体可用于噬菌体治疗。