Abstract To promote and enhance the biological responses of the implant hydroxyapatite (HAP) coatings these are modified by substitution with various ions for the development of bioactive bone implants. In the present work, we have fabricated a MgO/Tb,Eu-HAP dual substituted hydroxyapatite coating on borate passivated 316L stainless steel (316L SS) by the electrodeposition method. The MgO/Tb,Eu-HAP dual substituted hydroxyapatite coating on borate passivated 316L SS has been characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffractometry (XRD), high resolution scanning electron microscopy (HRSEM) with an energy dispersive X-ray (EDAX) analysis. Also, the mechanical property of the MgO/Tb,Eu-HAP dual layer coating has been evaluated using adhesion and Vickers micro-hardness tests. The corrosion performance of MgO/Tb,Eu-HAP dual layer coating has been investigated in Ringer's solution. The antibacterial activity of dual layer coating was evaluated against Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria) bacterial strains. The alkaline phosphate activity of MC3T3-E1 osteoblasts on the MgO/Tb,Eu-HAP dual layer coating was tested in order to examine their differentiation phase which is a quantitative marker of osteogenesis. In addition, the in-vitro biocompatibility of cell proliferation and Live/Dead assay of MgO/Tb,Eu-HAP dual layer coating were investigated on MC3T3-E1 cells. Hence these results improved the anti-corrosion performance, bioactivity and biocompatibility of MgO/Tb,Eu-HAP coating on passivated 316L SS as well. Finally, it is concluded that the MgO/Tb,Eu-HAP dual layer coating can be considered to be a capable candidate for biomedical applications.

中文翻译：

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Smart Flower Like Mgo/Tb、Eu 取代的羟基磷灰石双层涂层在 316l Ss 上增强了耐腐蚀性、抗菌活性和骨相容性

摘要 为了促进和增强植入物羟基磷灰石 (HAP) 涂层的生物反应，通过用各种离子取代这些涂层来开发生物活性骨植入物。在目前的工作中，我们通过电沉积方法在硼酸盐钝化的 316L 不锈钢（316L SS）上制备了 MgO/Tb,Eu-HAP 双取代羟基磷灰石涂层。硼酸盐钝化 316L SS 上的 MgO/Tb,Eu-HAP 双取代羟基磷灰石涂层已通过傅里叶变换红外 (FT-IR) 光谱、X 射线衍射 (XRD)、高分辨率扫描电子显微镜 (HRSEM) 与能量色散 X 射线 (EDAX) 分析。此外，MgO/Tb,Eu-HAP 双层涂层的机械性能已使用附着力和维氏显微硬度测试进行评估。MgO/Tb的腐蚀性能，已经在林格溶液中研究了 Eu-HAP 双层涂层。评估了双层涂层对金黄色葡萄球菌（革兰氏阳性菌）和大肠杆菌（革兰氏阴性菌）菌株的抗菌活性。测试了 MC3T3-E1 成骨细胞对 MgO/Tb、Eu-HAP 双层涂层的碱性磷酸盐活性，以检查它们的分化阶段，这是成骨的定量标志物。此外，在 MC3T3-E1 细胞上研究了细胞增殖的体外生物相容性和 MgO/Tb、Eu-HAP 双层涂层的活/死测定。因此，这些结果也提高了钝化 316L SS 上 MgO/Tb,Eu-HAP 涂层的防腐性能、生物活性和生物相容性。最后得出结论，MgO/Tb，