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Efficient Micropropagation of Chestnut Hybrids (Castanea spp.) Using Modified Woody Plant Medium and Zeatin Riboside
Horticultural Plant Journal ( IF 5.7 ) Pub Date : 2020-09-12 , DOI: 10.1016/j.hpj.2020.09.006
Guoqing Song , Qiuxia Chen , Pete Callow , Mario Mandujano , Xiaoyan Han , Beatriz Cuenca , Gregory Bonito , Carmen Medina-Mora , Dennis Wayne Fulbright , Daniel E. Guyer

The chestnuts genus (Castanea spp.) is comprised of economically important trees native to the Northern hemisphere that are used as food and hardwood timber. Here, a very efficient method for micropropagation of European × Japanese chestnut hybrids (Castanea sativa × C. crenata) is described. Woody Plant Medium was used as the basal medium. In vitro shoots of four rootstock cultivars were micropropagated without shoot-tip necrosis on multiplication medium containing 5.7 or 11.4 µmol·L−1 zeatin riboside, and were rooted on rooting medium containing 2.46 µmol·L−1 indolebutyric acid. Monthly shoot multiplication rates for each cultivar were 2–5 folds. In vitro rooting percentages for four cultivars were 87% for ‘Maraval’, 67% for ‘Marigoule’, 93% for ‘Marsol’, and 97% for ‘Précoce Migoule’. Within a 5 week period, 80%–95% of rooted shoots were successfully acclimated under high humidity conditions after they were planted in either soil or rockwool.



中文翻译:

改良木本植物培养基和玉米素核苷对板栗杂种()的高效微繁

栗属(Castanea spp。)由北半球原生的重要经济树木组成,用作食品和硬木木材。在此,描述了一种非常有效的欧洲×日本板栗杂交种(栗栗 × 弯孢)微繁殖的方法。木本植物培养基用作基础培养基。含有5.7或11.4 µmol·L -1玉米素核糖核苷的繁殖培养基上微繁殖4个砧木品种的新芽,而没有梢尖坏死,并在含有2.46 µmol·L -1吲哚丁酸的生根培养基上生根。每个品种的月芽繁殖率是2到5倍。体外四个品种的生根率分别为“ Maraval”为87%,“ Marigoule”为67%,“ Marsol”为93%,“PrécoceMigoule”为97%。在种植土壤或岩棉后的5周内,高根条件下成功地使80%–95%的生根芽适应了环境。

更新日期:2020-09-12
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