Objective

The aim of the present study was to evaluate the effect of quercetin on the acid resistance of human dentin through both laboratory and clinical studies.

Methods

Two hundred and twelve dentin blocks (2 mm × 2 mm × 2 mm) were prepared and used. For the laboratory study, dentin specimens were randomly divided into 8 groups (n = 12): deionized water, ethanol, 1.23 × 10⁴ μg/ml sodium fluoride (NaF), 120 μg/ml chlorhexidine, 183.2 μg/ml epigallocatechin gallate (EGCG), and 75 μg/ml, 150 μg/ml, and 300 μg/ml quercetin (Q75, Q150, and Q300). The specimens were treated with the respective solutions for 2 min and then subjected to in vitro erosion (4 cycles/d for 7 d). The surface microhardness loss (%SMH_l), erosive dentin wear, and surface morphology were evaluated and compared. For the impact on MMP inhibition, the release of crosslinked carboxyterminal telopeptide of type I collagen (ICTP) and the thickness of the demineralized organic matrix (DOM) were measured using additional dentin specimens. For the clinical study, the specimens were treated with NaF or Q300 for 2 min and then subjected to in vivo erosion (4 cycles/d for 7 d). The %SMH_l and erosive dentin wear of the specimens were measured to determine whether quercetin similarly inhibits erosion in situ.

Results

The quercetin-treated group had a significantly lower %SMH_l and erosive dentin wear than any other group, and the effect was concentration-dependent in vitro (P < 0.05). Dentin treated with quercetin produced significantly less ICTP and had a thicker DOM than the control dentin (P < 0.05). After in vivo erosion, the %SMH_l and erosive dentin wear of the Q300 group were significantly lower than those of the control group (P < 0.05).

Significance

The application of quercetin was shown, for the first time, to increase the acid resistance of human dentin, possibly through MMP inhibition and DOM preservation.

中文翻译：

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槲皮素减少侵蚀性牙本质的磨损：来自实验室和临床研究的证据。

目的

本研究的目的是通过实验室和临床研究来评估槲皮素对人牙本质的耐酸性的影响。

方法

准备并使用了212个牙本质块（2 mm×2 mm×2 mm）。为实验室研究中，牙本质标本随机分成8组（n = 12）：去离子水，乙醇，1.23×10 ⁴微克/毫升氟化钠（NAF），120微克/毫升的氯己定，183.2微克/毫升表儿茶素没食子酸酯（ EGCG），75μg/ ml，150μg/ ml和300μg/ ml槲皮素（Q75，Q150和Q300）。将标本分别用溶液处理2分钟，然后进行体外腐蚀（4个循环/天，共7天）。表面显微硬度损失（％SMH _l），侵蚀性牙本质磨损和表面形态进行了评估和比较。对于MMP抑制的影响，使用其他牙本质标本测量了I型胶原交联的羧基末端端肽（ICTP）的释放和脱矿质有机基质（DOM）的厚度。对于临床研究，将标本用NaF或Q300处理2分钟，然后进行体内腐蚀（4个循环/天，共7天）。所述％SMH_升和样品的侵蚀性磨损牙本质测量，以确定是否槲皮素类似地抑制侵蚀原位。

结果

槲皮素治疗组有显著下部％SMH_升和侵蚀牙本质磨损比任何其他基团，并且该效果浓度依赖性体外（P <0.05）。用槲皮素处理的牙本质产生的ICTP明显少于对照牙本质（P <0.05）。后在体内侵蚀时，％SMH_升和Q300组的冲蚀磨损牙本质均显著低于对照组（的P <0.05）。

意义

槲皮素的应用首次显示出可能通过抑制MMP和DOM来增加人牙本质的耐酸性。