Biochemical and Biophysical Research Communications ( IF 2.985 ) Pub Date : 2020-09-11 , DOI: 10.1016/j.bbrc.2020.09.018 Sundararaj S Jeremiah,Kei Miyakawa,Takeshi Morita,Yutaro Yamaoka,Akihide Ryo
The pandemic of COVID-19 is spreading unchecked due to the lack of effective antiviral measures. Silver nanoparticles (AgNP) have been studied to possess antiviral properties and are presumed to inhibit SARS-CoV-2. Due to the need for an effective agent against SARS-CoV-2, we evaluated the antiviral effect of AgNPs. We evaluated a plethora of AgNPs of different sizes and concentration and observed that particles of diameter around 10 nm were effective in inhibiting extracellular SARS-CoV-2 at concentrations ranging between 1 and 10 ppm while cytotoxic effect was observed at concentrations of 20 ppm and above. Luciferase-based pseudovirus entry assay revealed that AgNPs potently inhibited viral entry step via disrupting viral integrity. These results indicate that AgNPs are highly potent microbicides against SARS-CoV-2 but should be used with caution due to their cytotoxic effects and their potential to derange environmental ecosystems when improperly disposed.
由于缺乏有效的抗病毒措施，COVID-19的大流行没有得到控制。银纳米颗粒（AgNP）已被研究具有抗病毒特性，并被认为具有抑制SARS-CoV-2的作用。由于需要有效的抗SARS-CoV-2药物，我们评估了AgNPs的抗病毒作用。我们评估了许多不同大小和浓度的AgNPs，观察到直径约10 nm的颗粒在浓度范围为1至10 ppm时可有效抑制细胞外SARS-CoV-2，而在浓度为20 ppm或更高时观察到细胞毒性作用。基于萤光素酶的假病毒进入试验表明，AgNPs通过破坏病毒完整性来有效抑制病毒进入步骤。