To reach the goal of large-scale seaweed cultivation in Norway, new knowledge concerning commercially important species like the kelp Saccharina latissima is essential. This includes fundamental understanding of physiological mechanisms like nutrient uptake kinetics to better understand its ecological niche and nutritional requirements. The initial short-term nitrate (NO₃⁻) uptake kinetics in cultivated S. latissima juvenile sporophytes were evaluated under nitrogen-saturated and nitrogen-limited conditions. The uptake was measured for concentrations in a gradient from 2 to 18 μM NO₃⁻ which is representative of Norwegian coastal waters in the main growth season of winter and early spring. Preconditioning treatments led to internal nitrogen pools (total tissue nitrogen and intracellular nitrate) that were significantly lower for the N-limited than for the N-saturated sporophytes prior to the experiment. Nitrate uptake rates, related to biomass (V) and intracellular nitrogen content (U), were linearly related to the substrate concentrations for both N-limited and N-saturated sporophytes, indicating that S. latissima requires high ambient nitrate concentrations to maintain rapid growth. The sporophytes with deficient internal nitrogen pools exhibited higher uptake rates of NO₃⁻ than sporophytes with higher internal pools of nitrogen. Mathematical modelling was used to investigate the temporal development of total tissue nitrogen (Q_N) based on the nitrogen-specific uptake rates (U) and revealed a near linear response of U to changes in Q_N. The model also found that a maximum estimated value for Q_N was only approached after more than 20 days at external NO₃- concentrations of 8 μM. These results expand the physiological understanding of cultivated S. latissima and are important for a sustainable upscaling of seaweed farm production.

为了达到挪威大规模种植海藻的目标，有关商业上重要的物种（如海带Saccharina latissima）的新知识至关重要。这包括对生理机制（例如养分吸收动力学）的基本了解，以更好地了解其生态位和营养需求。初始短期硝酸盐（NO ₃ ^- ）的吸收动力学栽培S. latissima少年孢子体氮饱和和氮受限的条件下进行了评价。摄取测量了浓度梯度2至18μMNO ₃ ^-在冬季和初春的主要生长季节，它是挪威沿海水域的代表。预处理处理导致内部氮库（组织中的总氮和细胞内硝酸盐）的含量，在进行实验前，N受限的氮含量显着低于N饱和的子孢子。氮吸收速率与生物量（V）和细胞内氮含量（U）有关，与N限制和N饱和孢子体的底物浓度呈线性关系，这表明S. latissima需要较高的环境硝酸盐浓度才能保持快速生长。 。与缺乏内部氮气池孢子体没有表现出的较高的吸收速率₃ ^-比具有较高内部氮库的孢子体 数学模型用于基于氮的特定吸收率（U）研究总组织氮（Q _N）的时间变化，并揭示了U对Q _N变化的近线性响应。该模型还发现，仅在外部NO _3-浓度为8μM的情况下，超过20天后才达到Q _N的最大估计值。这些结果扩大了对栽培葡萄球菌的生理学认识，对于可持续提高海藻养殖场产量具有重要意义。