Angiostrongylus cantonensis is the main cause of human eosinophilic meningitis. Humans are accidental hosts, becoming infected due to ingestion of raw intermediate (snails and slugs) or paratenic hosts. Once ingested, the larvae migrate towards the brain where they die, causing the disease. To develop better mollusk control strategies, it is important to first understand what happens in the snail during infection, therefore our purpose was to characterize proteomic, metabolic and immunologic changes in Biomphalaria glabrata 24 h after infection with A. cantonensis. For this purpose, proteins were extracted from infected and uninfected snails and analyzed through mass spectrometry. Hemolymph was also collected, the number of hemocytes was counted and urea, nitric oxide, calcium, glycogen levels as well as alanine and aspartate aminotransferases activities were assessed. The cephalopodal region and gonad-digestive gland complex were dissected and their glycogen content was measured. After infection with A. cantonensis, we observed an increase of hemocytes and granulocytes as well as an increase in hemoglobin type 2 proteins. Temptin-like protein was also found up-regulated in infected snails. Several proteins with structural function (such as myosin heavy chain – striated muscle – like and protein LOC106059779 with ADAM/reprosolin domain) were also differentially expressed, suggesting loss/damage of internal tissues. Increase in phosphoglycerate mutase indicates an increase in glycolysis, possible to compensate the increase in energetic needs. Consequently, there is a decrease in glycogen reserves, particularly in the gonad – digestive gland complex.

广东圆管圆线虫是人类嗜酸性粒细胞性脑膜炎的主要原因。人类是偶然的宿主，由于摄入原始的中间体（蜗牛和）或腹泻的宿主而被感染。幼虫一旦被摄入，就会向着死亡的大脑迁移，从而引起疾病。为了制定更好的软体动物控制策略，首先要了解蜗牛在感染过程中发生的变化，这一点很重要，因此，我们的目的是鉴定被广州香椿菌感染后24 h 光滑小球藻的蛋白质组学，代谢和免疫学变化。为此，从感染和未感染的蜗牛中提取蛋白质，并通过质谱分析。还收集血淋巴，计数血细胞数目，并评估尿素，一氧化氮，钙，糖原水平以及丙氨酸和天冬氨酸转氨酶活性。解剖头足类区域和性腺消化腺复合体，并测定其糖原含量。感染广州曲霉后，我们观察到血细胞和粒细胞的增加以及2型血红蛋白的增加。在感染的蜗牛中也发现了类似蛋白的蛋白。还表达了几种具有结构功能的蛋白质（例如肌球蛋白重链–横纹肌–样蛋白和具有ADAM /视神经梭蛋白结构域的LOC106059779蛋白），表明内部组织丢失/损坏。磷酸甘油酸变位酶的增加表明糖酵解的增加，有可能弥补能量需求的增加。因此，糖原储备减少了，特别是在性腺-消化腺复合物中。