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Doping and Incorporation of Hydroxyapatite in Development of PU-PLA Electrospun Osteogenic Membranes
Journal of Polymers and the Environment ( IF 5.3 ) Pub Date : 2020-07-21 , DOI: 10.1007/s10924-020-01764-1
Waleed Mustafa , Usaid Azhar , Sobia Tabassum , Muddasar Jamal , Saadat Anwar Siddiqi , Muhammad Tariq , Nawshad Muhammad , Anila Asif , Aqif Anwar Chaudhry , Faiza Sharif

Complete restoration of bones to treat oral and craniofacial skeletal disorders, presents a challenge for orthopedic and maxillofacial experts. In the present research hydroxyapatite (HA) the main constituent of bone, has been modified with various dopants to enhance the biological as well as mechanical properties of skeletal tissue. The doped bioactive HA powder has been incorporated in the polymeric matrix of polyurethane (PU) and poly l lactic acid (PLLA) to fabricate osteogenic membranes. Four different composites were fabricated with dopants such as Sr, Mg, SiO4, and CO3 and were named as PU-PLA-Sr-HA, PU-PLA-Mg-HA, PU-PLA-Si-HA and PU-PLA-C-HA. The prepared osteogenic membranes were characterized using FTIR, XRD and EDX analyses. In vitro characterization was done to evaluate cell proliferation and calcium deposition using the MC3T3 pre-osteoblast cell line on the membranes. The mechanical properties i.e. tensile strength was also evaluated with respect to dopant types and their concentration. FTIR, XRD and EDX spectral analyses confirm the various dopings in the prepared HA powder. PU-PLA-Sr-HA membranes exhibit porous fibers with uniformly distributed pores along the surface of fibers. The tensile strength of membranes containing doped HA was higher than the membrane without adding HA powder. The Young’s modulus of PU-PLA-Mg-HA and PU-PLA-Si-HA membranes was higher compared to the other samples. Lowest Young’s modulus value was observed in the PU-PLA-Sr-HA membrane. Cell viability assay displayed very high biocompatibility for all the prepared membranes, specifically PU-PLA-C-HA and PU-PLA-Sr-HA membranes showed higher cell proliferation. Moreover, the doped HA incorporated membranes showed increased deposition of calcium through the Alizarin Red assay. In conclusion the electrospun fibrous membranes with doped HA displayed improved osteogenic properties and hence can be an excellent candidate for bone and dental regenerative applications.



中文翻译:

在PU-PLA电纺成骨膜的开发中掺入和掺入羟基磷灰石

彻底修复骨骼以治疗口腔和颅面骨骼疾病,对整形外科和颌面部专家提出了挑战。在本研究中,已使用各种掺杂剂对骨骼的主要成分羟基磷灰石(HA)进行了改性,以增强骨骼组织的生物学和机械性能。生物活性HA粉末的掺杂已聚氨酯(PU)和聚的聚合物基质被掺入乳酸(PLLA)以制造成骨膜。用诸如Sr,Mg,SiO 4和CO 3的掺杂剂制造了四种不同的复合材料分别命名为PU-PLA-Sr-HA,PU-PLA-Mg-HA,PU-PLA-Si-HA和PU-PLA-C-HA。使用FTIR,XRD和EDX分析对制备的成骨膜进行表征。使用膜上的MC3T3前成骨细胞系进行了体外表征,以评估细胞增殖和钙沉积。还针对掺杂剂类型及其浓度评估了机械性能,即拉伸强度。FTIR,XRD和EDX光谱分析证实了制备的HA粉中的各种掺杂。PU-PLA-Sr-HA膜的多孔纤维沿纤维表面均匀分布。包含掺杂的HA的膜的拉伸强度高于不添加HA粉末的膜。与其他样品相比,PU-PLA-Mg-HA和PU-PLA-Si-HA膜的杨氏模量更高。在PU-PLA-Sr-HA膜中观察到最低的杨氏模量值。细胞活力测定显示所有制备的膜都具有很高的生物相容性,特别是PU-PLA-C-HA和PU-PLA-Sr-HA膜显示出更高的细胞增殖能力。此外,掺入HA的掺杂膜通过茜素红分析显示钙的沉积增加。总之,掺有HA的电纺纤维膜显示出改善的成骨特性,因此可以成为骨骼和牙齿再生应用的极佳候选者。通过茜素红分析,掺入HA的掺杂膜显示钙的沉积增加。总之,掺有HA的电纺纤维膜显示出改善的成骨特性,因此可以成为骨骼和牙齿再生应用的极佳候选者。通过茜素红分析,掺入HA的掺杂膜显示钙的沉积增加。总之,掺有HA的电纺纤维膜显示出改善的成骨特性,因此可以成为骨骼和牙齿再生应用的极佳候选者。

更新日期:2020-09-12
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