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“Bacterial consortium from hydrothermal vent sediments presents electrogenic activity achieved under sulfate reducing conditions in a microbial fuel cell”
Journal of Environmental Health Science and Engineering ( IF 3.4 ) Pub Date : 2020-09-11 , DOI: 10.1007/s40201-020-00537-1
Margarita Isabel Pérez-Díaz 1 , Paola Zárate-Segura 2 , Luis Antonio Bermeo-Fernández 1 , Khemlal Nirmalkar 3 , Fernando Bastida-González 2 , Jaime García-Mena 3 , Janet Jan-Roblero 4 , Claudia Guerrero-Barajas 1
Affiliation  

Purpose

The aim of the present work was to assess the electrogenic activity of bacteria from hydrothermal vent sediments achieved under sulfate reducing (SR) conditions in a microbial fuel cell design with acetate, propionate and butyrate as electron donors.

Methods

Two different mixtures of volatile fatty acids (VFA) were evaluated as the carbon source at two chemical oxygen demand (COD) proportions. The mixtures of VFA used were: acetate, propionate and butyrate COD: 3:0.5:0.5 (stage 1) and acetate – butyrate COD: 3.5:0.5 (stage 2). Periodical analysis of sulfate (SO4−2), sulfide (HS) and COD were conducted to assess sulfate reduction (SR) and COD removal along with measurements of voltage and current to assess the global performance of the consortium in the system.

Results

Percentage of SR was of 97.5 ± 0.7 and 74.3 ± 1.5% for stage 1 and 2, respectively. The % COD removal was of 91 ± 2.1 and 75.3 ± 9.6 for stage 1 and 2, respectively. Although SR and COD removal were higher at stage 1, in regards of energy, stage 2 presented higher current and power densities and Coulombic efficiency as follows: 741.7 ± 30.5 μA/m2, 376 ± 34.4 μW/m2 and 5 ± 2.7%, whereas for stage 1 these values were: 419 ± 71 μA/m2, 52.7 ± 18 μW/m2 and 0.02%, respectively. A metagenomic analysis – stage 2 – in the anodic chamber, demonstrated that SR was due to Dethiosulfovibrionaceae (HA73), Desulfobacter and Desulfococcus and the electrogenic microorganisms were Planococcus, SHD-231, Proteiniclasticum, vadinCA02, and families Porphyromonadacea and Pseudomonadaceae.

Conclusions

It was demonstrated that microorganisms prevenient from hydrothermal vent sediments adapted to a microbial fuel cell system are able to generate electricity coupled to 74.3 ± 1.5 and 75.3 ± 9.6% of SR and COD removal respectively, with a mixture of acetate – butyrate.



中文翻译:

“来自热液喷口沉积物的细菌聚生体呈现出在微生物燃料电池中硫酸盐还原条件下实现的生电活动”

目的

本工作的目的是评估在硫酸盐还原 (SR) 条件下以乙酸盐、丙酸盐和丁酸盐作为电子供体的微生物燃料电池设计中热液喷口沉积物中细菌的电活性。

方法

两种不同的挥发性脂肪酸 (VFA) 混合物被评估为两种化学需氧量 (COD) 比例的碳源。使用的 VFA 混合物为:乙酸盐、丙酸盐和丁酸盐 COD:3:0.5:0.5(第 1 阶段)和乙酸盐 - 丁酸盐 COD:3.5:0.5(第 2 阶段)。对硫酸盐 (SO 4 -2 )、硫化物 (HS - ) 和 COD 进行定期分析以评估硫酸盐还原 (SR) 和 COD 去除率,同时测量电压和电流以评估系统中联合体的整体性能。

结果

第一阶段和第二阶段的 SR 百分比分别为 97.5 ± 0.7 和 74.3 ± 1.5%。第 1 阶段和第 2 阶段的 % COD 去除率分别为 91 ± 2.1 和 75.3 ± 9.6。虽然 SR 和 COD 去除在第 1 阶段更高,但在能量方面,第 2 阶段呈现更高的电流和功率密度以及库仑效率,如下所示:741.7 ± 30.5 μA/m 2、376 ± 34.4 μW/m 2和 5 ± 2.7% ,而对于第 1 阶段,这些值分别为:419 ± 71 μA/m 2、52.7 ± 18 μW/m 2和 0.02%。在阳极室中进行的第 2 阶段宏基因组分析表明,SR 是由脱硫硫弧菌科( HA73 )、脱硫杆菌脱硫球菌引起的产电微生物为PlanococcusSHD-231ProteniclasticumvadinCA02PorphyromonadaceaPseudomonadaceae

结论

结果表明,适应于微生物燃料电池系统的热液喷口沉积物中的微生物能够产生电力,分别与乙酸丁酸混合物的 74.3 ± 1.5 和 75.3 ± 9.6% 的 SR 和 COD 去除率相关。

更新日期:2020-09-12
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