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Genome-wide identification, characterization and expression analysis of non-RD receptor like kinase gene family under Colletotrichum truncatum stress conditions in hot pepper
Genetica ( IF 1.5 ) Pub Date : 2020-09-12 , DOI: 10.1007/s10709-020-00104-4
R Srideepthi 1 , M S R Krishna 1 , P Suneetha 2 , R Sai Krishna 3 , S Karthikeyan 1
Affiliation  

Receptor like kinases (RLKs) are preserved upstream signaling molecules which regulate several biological processes from plant development to various stress adaptation programs. Non arginine aspartate (non-RD) a prominent class of RLKs plays a significant role in disease resistance and apoptosis in plants. In present investigation, a comprehensive in silico analysis for non-RD Kinase gene family as well as identification of gene structures, sequence similarity, chromosomal localization, gene duplication analysis, promoter analysis, transcript expression profiles and phylogenic studies were done. In this study, twenty-six genes were observed on nine out of twelve chromosomes. All these genes were clustered into five subfamilies under large monophyletic group termed as Interleukin-1 Receptor-Associated Kinase (IRAK) family. Some of the important physiochemical properties of twenty-six proteins are determined and ranged in the following order: (a) Amino acids size ranged from (620 to 1781) (b) Molecular weight ranged as of (70.11 to 197.11 KDa) and (c) Theoretical PI ranged from (5.69 to 8.63) respectively. Structural diversity in genomic structure among non-RD kinase gene family was identified and presence of pathogen induced cis regulatory elements including STRE, MYC, MYB, and W box were found. Expression profiles revealed the potential ability of three genes CaRLK1 from LRRXII and CaRLK15,16 from stress antifung subfamily were pointedly upregulated beyond the severe stress time period (9 DAI) in anthracnose resistant genotype PBC-80 in response to Colletotrichum truncatum infection. Subsequently, in silico studies from the available genome sequencing data helped us to identify candidate genes tangled in inducing disease resistance.

中文翻译:

锥状炭疽菌胁迫条件下辣椒非RD受体样激酶基因家族的全基因组鉴定、表征及表达分析

受体样激酶 (RLK) 保留在上游信号分子中,可调节从植物发育到各种胁迫适应程序的多种生物过程。非精氨酸天冬氨酸 (non-RD) 是一类突出的 RLK,在植物的抗病性和细胞凋亡中起重要作用。在目前的调查中,对非 RD 激酶基因家族进行了全面的计算机分析,并进行了基因结构、序列相似性、染色体定位、基因重复分析、启动子分析、转录表达谱和系统发育研究的鉴定。在这项研究中,在 12 条染色体中的 9 条上观察到了 26 个基因。所有这些基因都聚集在称为白细胞介素 1 受体相关激酶 (IRAK) 家族的大型单系群下的五个亚家族中。确定了 26 种蛋白质的一些重要理化特性,并按以下顺序排列:(a) 氨基酸大小范围从 (620 到 1781) (b) 分子量范围从 (70.11 到 197.11 KDa) 和 (c ) 理论 PI 范围分别为 (5.69 至 8.63)。鉴定了非 RD 激酶基因家族中基因组结构的结构多样性,并发现了病原体诱导的顺式调控元件,包括 STRE、MYC、MYB 和 W 盒的存在。表达谱揭示了来自 LRRXII 的三个基因 CaRLK1 和来自胁迫抗真菌亚科的 CaRLK15,16 的潜在能力在炭疽病抗性基因型 PBC-80 中超过严重胁迫时间段 (9 DAI) 后被明确上调,以响应截尾炭疽菌感染。随后,
更新日期:2020-09-12
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