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Establishment of the hairy root culture of Echium plantagineum L. and its shikonin production.
3 Biotech ( IF 2.8 ) Pub Date : 2020-09-11 , DOI: 10.1007/s13205-020-02419-7
Jiang-Yan Fu 1 , Hua Zhao 1 , Jia-Xin Bao 1 , Zhong-Ling Wen 1 , Rong-Jun Fang 1 , Aliya Fazal 1 , Min-Kai Yang 1 , Bao Liu 2 , Tong-Ming Yin 3 , Yan-Jun Pang 1, 3 , Gui-Hua Lu 1, 3, 4 , Jin-Liang Qi 1, 3 , Yong-Hua Yang 1, 3
Affiliation  

Echium plantagineum L. (Boraginaceae) is an invasive species in Australia and contains medicinal shikonins in its roots. In this study, the hairy root lines of E. plantagineum were established using Agrobacterium rhizogenes strain ATCC15834 and confirmed by the amplification of the rolB gene. Results showed significant difference in shikonin production between the hairy root lines in the 1/2B5 and M9 media. The biomass of the lines in the 1/2B5 medium was fivefold of that in the M9 medium. However, the components of detected shikonins were similar in these two liquid media. By contrast, different accumulation profiles appeared in the hairy root lines. HPLC analysis revealed the presence of nine possible related compounds, including shikonins, and acetylshikonin was the most abundant shikonin derivative. The content of acetylshikonin in the 1/2B5 medium (36.25 mg/L on average) was twofold of that in the M9 medium. Our results showed that the hairy root cultures of E. plantagineum can be used in enhancing the production of potential pharmaceutical compounds, such as acetylshikonin.



中文翻译:

车前草毛根培养的建立及紫草素的生产。

Echium plantagineum L.(紫草科)是澳大利亚的入侵物种,其根部含有药用紫草素。本研究采用发根农杆菌ATCC15834菌株建立前草毛根系,并通过rolB基因的扩增得到证实。结果显示1 / 2 B5 和 M9 培养基中毛状根系之间紫草素产量的显着差异。1 / 2系中的生物量B5培养基是M9培养基的五倍。然而,在这两种液体培养基中检测到的紫草素的成分相似。相比之下,毛状根系中出现了不同的积累曲线。HPLC 分析显示存在九种可能的相关化合物,包括紫草素,乙酰紫草素是最丰富的紫草素衍生物。1 / 2 B5培养基中乙酰紫草素的含量(平均36.25 mg/L)是M9培养基的两倍。我们的结果表明,车前草的毛状根培养物可用于提高潜在药物化合物(如乙酰紫草素)的产量。

更新日期:2020-09-12
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