Chemical probe‐based approaches have proven powerful in recent years in the target identification studies of natural products. OSW‐1 is a saponin class of natural products with highly potent and selective cytotoxicity against various cancer cell lines. Understanding its mechanism of action is important for the development of anticancer drugs with potentially novel target pathways. This account reviews recent progress in the development of OSW‐1 derived probes for exploring the mechanism of its action. The key to the probe development is a judicious choice of functionalization sites and a selective functionalization strategy. The types of probes include fluorescent probes for cellular imaging analysis and affinity probes for target identification analysis.

中文翻译：

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开发用于抗癌皂苷OSW-1功能分析的化学探针

近年来，在天然产物的目标识别研究中，基于化学探针的方法已被证明是有效的。OSW-1是一种天然的皂素类产品，对各种癌细胞具有高度有效的选择性细胞毒性。了解其作用机理对于开发具有潜在新型靶途径的抗癌药物很重要。此帐户回顾了OSW-1衍生探针在探索其作用机理方面的最新进展。探针开发的关键是明智选择功能化位点和选择性功能化策略。探针的类型包括用于细胞成像分析的荧光探针和用于靶标鉴定分析的亲和探针。