Abstract

A monolithic chromatographic column for HPLC with a sorbent based on 1-vinyl-1,2,4-triazole has been prepared for the first time. It has been shown that, depending on the composition of the mobile phase (acetonitrile/water ratio), this column is able to operate in the modes of both reverse phase chromatography and hydrophilic chromatography. The dependences of the retention factor on the volume fraction of acetonitrile in the mobile phase have been studied for substances of acidic and basic nature. An example of the separation of the components of the pharmaceutical product Askofen-P on the prepared column has been provided.

中文翻译：

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基于1-乙烯基-1,2,4-三唑的带吸附剂的整体柱用于亲水性HPLC

摘要

首次制备了具有基于1-乙烯基-1,2,4-三唑的吸附剂的HPLC整体色谱柱。已经表明，取决于流动相的组成（乙腈/水的比例），该柱能够以反相色谱和亲水色谱的模式操作。对于酸性和碱性物质，已经研究了保留因子对流动相中乙腈体积分数的依赖性。提供了在准备好的色谱柱上分离药品Askofen-P的成分的示例。