During our research, cellulose‐based test sheets containing copper and potassium humate were prepared and tested against several bacterial and fungal strains. The energy dispersive X‐ray spectrometry and attenuated total reflectance (ATR) Fourier‐transform infrared spectroscopy revealed that humate contributed to increase the copper content of the test sheets. The antimicrobial impact of test sheets on various fungi (Penicillium expansum, Wallemia sebi, and Aspergillus brasiliensis) and bacteria (genera Micrococcus, Enterococcus, Bacillus, Salmonella, Staphylococcus, Listeria genera) was tested using a modified agar diffusion‐based assay. On the basis of the disc diffusion results, the sheets effectively inhibited all the tested fungi and Gram‐positive bacteria. The highest antimicrobial effect was exhibited by the copper‐containing test sheet with 1:0.1 and 1:0.25 cellulose:humate weight ratio. Deterioration test with blackberries demonstrated the shelf life extending effect of the sheets.

中文翻译：

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腐殖酸铜纤维板的抗菌活性

在我们的研究过程中，制备了含有腐殖酸铜和钾的纤维素基测试纸，并针对几种细菌和真菌菌株进行了测试。能量色散X射线光谱法和衰减全反射率（ATR）傅立叶变换红外光谱法表明，腐殖酸盐有助于增加试片中的铜含量。试纸对各种真菌（扩展青霉菌，Wallemia sebi和巴西曲霉）和细菌（细球菌，肠球菌，芽孢杆菌，沙门氏菌，金黄色葡萄球菌，李斯特菌）的抗菌效果）已使用改良的琼脂扩散分析法进行了测试。根据椎间盘的扩散结果，这些薄片可有效抑制所有测试的真菌和革兰氏阳性细菌。纤维素：腐植酸酯重量比为1：0.1和1：0.25的含铜测试纸表现出最高的抗菌效果。用黑莓进行的劣化测试证明了片材的保质期延长效果。