Green synthesis of nanoparticles is an emerging field of nanotechnology, preferred over physical and chemical synthesis owing to their safety, cost-effectiveness nature, bio-compatibility, eco-friendly and scalable properties. The present study includes Seripheidium quettense aqueous extract mediated green synthesis, optimization of silver nanoparticles (Sq-AgNPs) and their pharmacological evaluations. Synthesis was done considering various optimization parameters including concentrations of AgNO₃, pH of the reaction mixture, extract to precursor ratio and temperature. Biosynthesis was carried out using our already optimized conditions. UV–visible spectrophotometer, FTIR, XRD and SEM analysis were used for characterization of Sq-AgNPs. The synthesized Sq-AgNPs (49.96–54.36 nm) were evaluated for their antibacterial, antifungal, anticancer and hemolytic potentials. The maximum antibacterial activity was found against Escherichia coli, Klebsiella pneumonia and Bacillus subtilis with their MICs of 11.1, 33.3 and 33.3 μg/mL, respectively. Aspergillus nigar was found as the most susceptible fungal strain with the highest zone of inhibition (13.2 ± 0.72 mm). Sq-AgNPs inhibited proliferation of human liver cancer cell lines (HepG2) with median lethal concentration (IC₅₀s) of 62.5 µg/mL. Results of the hemolytic assay showed that SqNPs are bio-compatible and have less effect on erythrocytes even at high concentration of 100 µg/mL.

纳米粒子的绿色合成是纳米技术的一个新兴领域，由于其安全性，成本效益，生物相容性，生态友好和可扩展的特性，因此优于物理和化学合成。本研究包括藜麦草提取物介导的绿色合成，银纳米颗粒（Sq-AgNPs）的优化及其药理评价。考虑各种优化参数（包括AgNO _3的浓度）进行合成，反应混合物的pH值，萃取液与前体的比例和温度。使用我们已经优化的条件进行生物合成。紫外可见分光光度计，FTIR，XRD和SEM分析用于表征Sq-AgNPs。评估了合成的Sq-AgNPs（49.96–54.36 nm）的抗菌，抗真菌，抗癌和溶血潜力。发现对大肠杆菌，肺炎克雷伯菌和枯草芽孢杆菌具有最大的抗菌活性，其MIC分别为11.1、33.3和33.3μg/ mL。黑曲霉被发现是最敏感的真菌菌株，具有最高的抑制区（13.2±0.72 mm）。Sq-AgNPs以62.5 µg / mL的中位致死浓度（IC ₅₀ s）抑制人肝癌细胞系（HepG2）的增殖。溶血分析结果表明，即使在100 µg / mL的高浓度下，SqNPs仍具有生物相容性，对红细胞的影响较小。