In this study, a novel solid phase extraction method was constructed to detect three tyrosine kinase inhibitors (TKIs) in urine with a high-performance liquid chromatography-diode array detector. The sorbent MCN/BIF-20 was constructed by magnetic g-C₃N₄ (MCN) and boron imidazole framework-20 (BIF-20) and was characterized by multiple techniques. The experimental results of the adsorption isotherm and adsorption kinetics indicated that the composites had good adsorption of TKIs (148.33 mg g⁻¹, 283.25 mg g⁻¹, 188.17 mg g⁻¹). The reason for the good adsorption property of the complex material was revealed by comparison with each single material. The analytical method was built by a single factor experiment, and was evaluated as a suitable method to detect TKIs in urine by its good accuracy (90.35–98.69%), precision (<3.9%), appropriate detection limits (2.2–3.4 ng mL⁻¹), and linear ranges (12.5–500 ng mL⁻¹) with convenient determination coefficients (>0.9997). The performance of the MCN/BIF-20 composite did not decrease dramatically in 3 cycles. These analytical results demonstrated that g-C₃N₄ and BIFs had a bright prospect in sample pretreatment, and the proposed approach based on MCN/BIF-20 was applicable for analysis of TKIs in urine.

中文翻译：

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固相萃取基磁性氮化碳/金属有机骨架复合材料与高效液相色谱法测定尿样中的酪氨酸激酶抑制剂。

在这项研究中，构造了一种新型的固相萃取方法，用高效液相色谱-二极管阵列检测器检测尿液中的三种酪氨酸激酶抑制剂（TKI）。MCN / BIF-20吸附剂由磁性gC ₃ N ₄（MCN）和咪唑硼骨架20（BIF-20）构成，并通过多种技术进行了表征。吸附等温线和吸附动力学的实验结果表明，该复合材料具有良好的吸附TKI的（148.33毫克克^-1，283.25毫克克^-1，188.17毫克克^-1）。通过与每种单一材料进行比较，揭示了复合材料具有良好吸附性能的原因。该分析方法是通过单因素实验建立的，并以其良好的准确度（90.35–98.69％），精密度（<3.9％），适当的检出限（2.2–3.4 ng mL）被评估为检测尿液中TKI的合适方法。^-1）和线性范围（12.5-500 ng mL ^-1）以及方便的测定系数（> 0.9997）。MCN / BIF-20复合材料的性能在3个循环中均未显着下降。这些分析结果表明，gC ₃ N ₄和BIFs在样品预处理中具有广阔的前景，所提出的基于MCN / BIF-20的方法可用于尿液中TKI的分析。