Background:

Circulating miRNAs have been proved to be promising biomarkers for disease detection in recent years. The present study aimed at exploring available serum miRNA biomarkers for the detection of colorectal cancer.

Methods:

A three-phase study was performed to select and validate candidate miRNAs with significant dysregulation in colorectal cancer using quantitative reverse transcription-polymerase chain reaction. This study recruited 137 colorectal cancer patients and 145 healthy controls. The diagnostic values of miRNAs were evaluated by receiver operating characteristic analysis. Bioinformatics analyses were utilized to predict target genes of miRNAs, and to conduct functional annotation and enrichment.

Results:

miR-30e-3p, miR-31-5p, miR-34b-3p and miR-146a-5p, miR-148a-3p and miR-192-5p were significantly dysregulated in colorectal cancer serum when compared with healthy controls. The panel composed of miR-30e-3p, miR-146a-5p, and miR-148a-3p exhibited strong diagnostic ability. The area under the receiver operating characteristic curve of the three-miRNA panel was 0.883, with a sensitivity of 0.800 and specificity of 0.787.

Conclusion:

The present study identified a three-miRNA panel in serum with a strong diagnostic ability of colorectal cancer, which may be able to serve as a novel noninvasive biomarker for colorectal cancer detection.

中文翻译：

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血清中的三 miRNA 面板作为结肠直肠癌检测的非侵入性生物标志物。

背景：

近年来，循环 miRNA 已被证明是一种很有前途的疾病检测生物标志物。本研究旨在探索可用于检测结直肠癌的血清 miRNA 生物标志物。

方法：

使用定量逆转录聚合酶链反应进行了一项三阶段研究，以选择和验证在结肠直肠癌中具有显着失调的候选 miRNA。本研究招募了 137 名结直肠癌患者和 145 名健康对照。通过接受者操作特征分析评估miRNA的诊断价值。生物信息学分析用于预测 miRNA 的靶基因，并进行功能注释和富集。

结果：

与健康对照相比，miR-30e-3p、miR-31-5p、miR-34b-3p 和 miR-146a-5p、miR-148a-3p 和 miR-192-5p 在结直肠癌血清中显着失调。由miR-30e-3p、miR-146a-5p和miR-148a-3p组成的panel表现出很强的诊断能力。三miRNA组的受试者工作特征曲线下面积为0.883，敏感性为0.800，特异性为0.787。

结论：

本研究在血清中发现了一个具有很强的结直肠癌诊断能力的三 miRNA panel，它可能能够作为一种新型的非侵入性生物标志物用于结直肠癌的检测。