Holstein steers (n = 16) were used to determine if a synthetic alkaloid, bromocriptine, would alter the transcriptome of the small intestine and adjacent mesenteric adipose. On d 0, steers were assigned to one of two treatments: control (CON; saline only) or bromocriptine (BROMO; 0.1 mg/kg BW bromocriptine mesylate injected intramuscularly every 3 d for 30 d). Steers were slaughtered and midpoint sections of jejunal epithelium and associated mesenteric fat were collected for RNA isolation. Transcriptome analysis was completed via RNA-Seq to determine if BROMO differed compared with CON within intestinal epithelium or mesenteric adipose mRNA isolates. Differential expression thresholds were set at a significant P-value (P < 0.05) and a fold change ≥ 1.5. Only two genes were differentially expressed within the intestinal epithelium but there were 20 differentially expressed genes in the mesenteric adipose tissue (six up regulated and 14 down regulated). Functions related to cell movement, cell development, cell growth and proliferation, cell death, and overall cellular function and maintenance were the top five functional molecular categories influenced by BROMO treatment within the intestinal epithelium. The top molecular categories within mesenteric adipose were antigen presentation, protein synthesis, cell death, cell movement, and cell to cell signaling and interaction. In conclusion, BROMO treatment influenced the intestinal epithelium and mesenteric adipose transcriptome and identified genes and pathways influential to the effects associated with alkaloid exposure which are important to beef production.

荷斯坦牛（ñ= 16）用于确定合成生物碱溴隐亭是否会改变小肠和邻近肠系膜脂肪的转录组。在第0天，将ste牛皮划归两种处理中的一种：对照（CON；仅盐水）或溴隐亭（BROMO； 0.1 mg / kg BW甲磺酸溴隐亭甲磺酸盐，每3天肌注30天）。屠宰ers牛，空肠上皮和相关的肠系膜脂肪的中点切片用于RNA分离。通过RNA-Seq完成转录组分析，以确定肠上皮或肠系膜脂肪mRNA分离物中的BROMO与CON相比是否有所不同。差异表达阈值设置为显着P值（P<0.05）且倍数变化≥1.5。肠上皮中只有两个基因差异表达，但肠系膜脂肪组织中有20个差异表达基因（六个上调和14个下调）。与细胞运动，细胞发育，细胞生长和增殖，细胞死亡以及整体细胞功能和维持有关的功能是受肠上皮内BROMO治疗影响的前五个功能分子类别。肠系膜脂肪中最重要的分子类别是抗原呈递，蛋白质合成，细胞死亡，细胞运动以及细胞间信号传导和相互作用。结论，