Abstract

Laccases (EC 1.10.3.2), a group of multi-copper oxidases (MCOs), play multiple biological functions and widely exist in many species. Fungal laccases have been extensively studied for their industrial applications, however, there was no database specially focused on fungal laccases. To provide a comparative genomics platform for fungal laccases, we have developed a comparative genomics platform for laccases and MCOs (http://laccase.riceblast.snu.ac.kr/). Based on protein domain profiles of characterized sequences, 3,571 laccases were predicted from 690 genomes including 253 fungi. The number of putative laccases and their properties exhibited dynamic distribution across the taxonomy. A total of 505 laccases from 68 genomes were selected and subjected to phylogenetic analysis. As a result, four clades comprised of nine subclades were phylogenetically grouped by their putative functions and analyzed at the sequence level. Our work would provide a workbench for putative laccases mainly focused on the fungal kingdom as well as a new perspective in the identification and classification of putative laccases and MCOs.

摘要

漆酶（EC 1.10.3.2）是一组多铜氧化酶（MCO），具有多种生物学功能，广泛存在于许多物种中。真菌漆酶已经对其工业应用进行了广泛的研究，但是，还没有专门针对真菌漆酶的数据库。为了提供用于真菌漆酶的比较基因组学平台，我们开发了用于漆酶和MCO的比较基因组学平台（http://laccase.riceblast.snu.ac.kr/）。根据特征序列的蛋白质结构域图谱，从690个基因组（包括253种真菌）中预测了3,571个漆酶。推定的漆酶的数量及其性质在整个分类学中表现出动态分布。从68个基因组中总共选择了505个漆酶，并进行了系统发育分析。结果是，将由9个子进化枝组成的4个进化枝按其假定功能进行系统发育分组，并在序列水平上进行分析。我们的工作将为主要针对真菌界的假定漆酶提供一个工作台，并为鉴定和分类假定漆酶和MCO提供新的视角。