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Bioprospecting of potential petroleum hydrocarbon degraders using bacterial strains isolated from soils around transformer installation areas
Energy Sources, Part A: Recovery, Utilization, and Environmental Effects ( IF 2.9 ) Pub Date : 2020-09-10 , DOI: 10.1080/15567036.2020.1815906
Nkem Torimiro 1 , Godswill E. Akhigbe 2, 3 , Festus M. Adebiyi 3
Affiliation  

The study assessed the contamination levels of the soils around transformer installation areas in Ile-Ife, which were contaminated with transformer oil. Contamination levels are determined by using some soil physicochemical properties (pH, electrical conductivity, and organic matter) and also identifying some hydrocarbon utilizing bacteria in the oil-contaminated soils. Soil samples were collected from transformer oil-contaminated and uncontaminated soils at 5−15-cm depth. Soil physicochemical properties were determined using standard methods. The soil samples were screened for hydrocarbon-degrading bacteria; the isolates were duly identified using the matrix-assisted laser desorption/ionization-time of flight/mass spectrometry technique. The growth rate of the bacterial isolates in the hydrocarbon medium was monitored. Thereafter, the residual oil was analyzed quantitatively and qualitatively using gravimetric and gas chromatography–mass spectrometry techniques, respectively. The results of the physiochemical parameters of the oil-contaminated soils showed that the soils had mean values of pH (5.01 ± 0.22), electrical conductivity (574.45 ± 63.35 µs/cm), and soil organic matter (50.53 ± 5.67 g/kg). The chromatogram of the residual oils from the biodegradation process showed the efficacy of Providencia stuartii and Bacillus pseudomycoides in degrading alkyl polycyclic aromatic hydrocarbons (PAHs), cycloalkanes, unsaturated alkanes, and unsaturated-branched alkanes, while Providencia rettgeri could adequately degrade cycloalkenes, cycloalkanes, unsaturated alkanes, and unsaturated-branched alkanes. In this study, novel hydrocarbon-degrading bacterial isolates were identified, and the extent of their hydrocarbon and complex organic compound utilization was shown, it suggests the need for further studies to explore their potentials in bioremediation programs.



中文翻译:

使用从变压器安装区周围土壤中分离出的细菌菌株对潜在的石油烃降解物进行生物勘探

该研究评估了Ile-Ife变压器安装区域周围土壤的污染水平,这些土壤被变压器油污染。通过使用一些土壤理化特性(pH,电导率和有机物),并在受油污染的土壤中鉴定一些利用碳氢化合物的细菌来确定污染水平。从5-15厘米深度的变压器油污染和未污染的土壤中收集土壤样品。使用标准方法确定土壤理化性质。筛选土壤样品中降解碳氢化合物的细菌;使用基质辅助激光解吸/电离-飞行时间/质谱技术适当地鉴定了分离物。监测细菌分离物在烃介质中的生长速率。之后,残留油分别采用重量分析法和气相色谱-质谱法进行了定性和定量分析。油污染土壤的理化参数结果表明,土壤的平均值为pH(5.01±0.22),电导率(574.45±63.35 µs / cm)和土壤有机质(50.53±5.67 g / kg) 。生物降解过程中残留油的色谱图显示了Providencia stuartii和Bacillus pseudomycoides可降解烷基多环芳烃(PAH),环烷烃,不饱和烷烃和不饱和支链烷烃,而Providetcia rettgeri可以充分降解环烯烃,环烷烃,不饱和烷烃和不饱和支链烷烃。在这项研究中,鉴定了新型的可降解碳氢化合物的细菌分离株,并显示了它们对碳氢化合物和复杂有机化合物的利用程度,这表明需要进一步研究以探索其在生物修复计划中的潜力。

更新日期:2020-09-11
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