G3: Genes, Genomes, Genetics ( IF 2.6 ) Pub Date : 2020-10-27 , DOI: 10.1534/g3.120.401689 Xiaocheng Yu 1 , Qiulin Qin 1 , Xia Wu 1 , Dandan Li 1 , Shengming Yang 2
In Medicago truncatula, some ecotypes form a black or purple stain in the middle of adaxial leaf surface due to accumulation of anthocyanins. However, this morphological marker is missing in some other ecotypes, although anthocyanin biosynthesis pathway is not disrupted. Genetic analysis indicated that the lack of the leaf spot of anthocyanins accumulation is a dominant trait, which is controlled by a single gene, LPP1. Genetic mapping indicated that the LPP1 gene was delimited to a 280 kb-region on Chromosome 7. A total of 8 protein-coding genes were identified in the LPP1 locus through gene annotation and sequence analysis. Of those, two genes, putatively encoding MYB-transcriptional suppressors, were selected as candidates for functional validation.
中文翻译:
Medi藜苜蓿叶片色素沉着模式基因的遗传和物理定位。
在Medi藜苜蓿中,由于花青素的积累,一些生态型在叶片的中叶表面形成黑色或紫色污渍。然而,尽管花色苷的生物合成途径没有被破坏,但在其他一些生态型中却缺少这种形态学标记。遗传分析表明,缺乏花色苷积累的叶斑是主要性状,其受单个基因LPP1控制。遗传作图表明,LPP1基因在7号染色体上被界定为一个280 kb的区域。在LPP1中共鉴定出8个蛋白质编码基因通过基因注释和序列分析找到基因座。其中,推定编码MYB转录抑制子的两个基因被选为功能验证的候选基因。