Pressurized hot water extraction (PHWE) could be a better alternative in enhancing the yield of valuable Citrus hystrix leaves essential oil. Thus, the fractional factorial design based on resolution V was used to assess the crucial factors that affect the essential oil yield (%Y) extracted from Citrus hystrix leaves through a PHWE. A maximum Y% of 1.63% was successfully isolated at conditions involving 0.08% moisture content, 1 mm particle size, 1:0.2 ml/g solvent‐to‐sample ratio, 30 min extraction time, and 120°C extraction temperature. Moisture content and particle size were the main effects that significantly affect the %Y, while the extraction time and solvent‐to‐sample ratio exhibited a moderate effect on %Y, followed by extraction temperature that showed the least effect on %Y. A developed regression model has shown good correlation and adequacy on %Y. Based on the compositional analysis, neoisopulegol hydrate appears to be the main constituents of the oil obtained.

中文翻译：

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基于分离度V分数分解设计筛选加压热水提取柑橘叶片精油产量的影响因素

加压热水提取（PHWE）可能是提高有价值的柑桔叶精油产量的更好选择。因此，基于分辨率V的分数阶乘设计用于评估影响通过PHWE从柑桔叶片中提取的香精油收率（％Y）的关键因素。在涉及0.08％水分，1 mm粒径，1：0.2 ml / g溶剂与样品的比率，30分钟提取时间和120°C提取温度的条件下，成功分离出最大Y％为1.63％。水分含量和粒径是显着影响％Y的主要影响，而萃取时间和溶剂-样品比对％Y表现出中等影响其次是提取温度对％Y的影响最小。发达的回归模型显示出与％Y的良好相关性和充分性。根据组成分析，新异胡薄荷醇水合物似乎是所得油的主要成分。