Tissue engineering is a method of growing importance regarding clinical application in the genitourinary region. One of the key factors in successfully development of an artificially tissue engineered mucosa equivalent (TEOM) is the optimal choice of the scaffold. Collagen scaffolds are regarded as gold standard in dermal tissue reconstruction. Four distinct collagen scaffolds were evaluated for the ability to support the development of an organotypical tissue architecture. TEOMs were established by seeding cocultures of primary oral epithelial cells and fibroblasts on four distinct collagen membranes. Cell viability was assessed by MTT‐assay. The 3D architecture and functionality of the tissue engineered oral mucosa equivalents were evaluated by confocal laser‐scanning microscopy and immunostaining. Cell viability was reduced on the TissuFoil E® membrane. A multi‐stratified epithelial layer was established on all four materials, however the TEOMs on the Bio‐Gide® scaffold showed the best fibroblast differentiation, secretion of tenascin and fibroblast migration into the membrane. The TEOMs generated on Bio‐Gide® scaffold exhibited the optimal cellular organization into a cellular 3D network. Thus, the Bio‐Gide® scaffold is a suitable matrix for engineering of mucosa substitutes in vitro.

中文翻译：

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用于泌尿生殖系统重建的全层组织工程口腔黏膜：不同胶原基可生物降解膜的比较

组织工程是一种在泌尿生殖区域临床应用中越来越重要的方法。成功开发人工组织工程粘膜等效物 (TEOM) 的关键因素之一是支架的最佳选择。胶原支架被认为是真皮组织重建的金标准。评估了四种不同的胶原蛋白支架支持器官型组织结构发展的能力。通过在四种不同的胶原膜上接种原代口腔上皮细胞和成纤维细胞的共培养物来建立 TEOM。通过 MTT 测定法评估细胞活力。通过共聚焦激光扫描显微镜和免疫染色评估了组织工程口腔粘膜等效物的 3D 结构和功能。TissuFoil E® 膜上的细胞活力降低。在所有四种材料上都建立了多层上皮层，但 Bio-Gide® 支架上的 TEOM 显示出最好的成纤维细胞分化、生腱蛋白分泌和成纤维细胞迁移到膜中。在 Bio-Gide® 支架上生成的 TEOM 将最佳的细胞组织呈现为细胞 3D 网络。因此，Bio-Gide® 支架是一种适合体外工程化黏膜替代物的基质。