Vanadium pentoxide (V₂O₅) was used as support material to immobilize the urease enzyme. V₂O₅ was prepared from a sol–gel process, and the immobilization of urease was done onto the surface of the PET/ITO/V₂O₅ film. Different concentrations of urea were tested to study the sensitivity of the biosensor. Tests with pH variation were carried out, and it was noticed that the total charge in the cyclic voltammograms decreases due to the increase in pH that influences the catalytic activity of the enzyme. From cyclic voltammograms, with a different scan rate, for linear correlation testing, it was observed that the anodic currents varied as a function of the scan rate. Based on the variation in the urea concentration, the current increased with the increase in the urea concentration, indicating the sensitivity of the material. The use of V₂O₅/urease showed favorable electrochemical responses for application in biosensors as well as being sensitive to the variation in the urea concentration and easy to obtain and prepare.

中文翻译：

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用于尿素生物传感器的 V2O5/尿素酶的合成与表征

五氧化二钒(V ₂ O ₅ )用作载体材料以固定脲酶。V ₂ O ₅由溶胶-凝胶法制备，脲酶固定在 PET/ITO/V ₂ O _{5 表面}电影。测试了不同浓度的尿素以研究生物传感器的灵敏度。进行了 pH 变化的测试，注意到循环伏安图中的总电荷由于影响酶催化活性的 pH 增加而减少。从具有不同扫描速率的循环伏安图中，对于线性相关测试，观察到阳极电流作为扫描速率的函数而变化。根据尿素浓度的变化，电流随着尿素浓度的增加而增加，表明材料的敏感性。V ₂ O ₅的用途/脲酶在生物传感器中表现出良好的电化学响应，并且对尿素浓度的变化很敏感，易于获得和制备。