Objectives

The main aim of this study is to isolate and characterize fibrinolytic enzyme from Bacillus flexus.

Methods

Fish meal of Sardinella longiceps and anchovy was optimized using a two-level full factorial design (2⁵) and response surface methodology. The significant physical factors and nutrient sources (peptone, maltose, and magnesium chloride) were identified by statistical approach. The properties of a purified enzyme including their effect at different temperature, pH and the effect of metal ions were evaluated.

Results

Enzyme yield was improved 3.5 fold than unoptimized medium. Central composite design optimized culture medium enhanced enzyme yield (4711 ± 29.3 U/g of substrate). The fibrinolytic enzyme was highly active at alkaline pH (8.0), 50 °C and the molecular weight was 32 kDa.

Conclusions

From these findings, it concludes that this fibrinolytic enzyme could be a novel potent thrombolytic agent.

中文翻译：

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来自海洋环境的新型弯曲芽孢杆菌产生的具有治疗潜力的纤溶酶的增强的生产，纯化和生化特性

目标

这项研究的主要目的是从弯曲芽孢杆菌中分离并鉴定纤溶酶。

方法

使用两级全因子设计（2 ⁵）和响应面方法优化了沙丁鱼和ic鱼的鱼粉。通过统计学方法确定了重要的物理因素和营养来源（蛋白p，麦芽糖和氯化镁）。评价了纯化酶的性质，包括它们在不同温度，pH和金属离子作用下的作用。

结果

酶产量比未优化的培养基提高了3.5倍。中央复合设计优化了培养基，提高了酶的产量（4711±29.3 U / g底物）。纤溶酶在碱性pH（8.0），50°C下具有很高的活性，分子量为32 kDa。

结论

从这些发现，可以得出结论，该纤溶酶可能是新型有效的血栓溶解剂。