HClO is an important signaling species, which is closely related to many diseases and physiological activities of the organism, and it plays an important role in the immune system. Therefore, it is of great significance to accurately and quickly monitor the HClO in the living organisms. However, in vivo detection of endogenous HClO remains a challenge. Herein, a near-infrared fluorescent probe CyClOP for the rapid detection of HClO was developed. CyClOP possesses several advantages such as fast response, good selectivity and deep penetration. CyClOP was successfully applied to the detection of endogenous HClO in living cells. In addition, CyClOP effectively captured the fluctuation of endogenous HClO produced by the immune system during infection with Staphylococcus aureus and muscle tissue injury in living mice for the first time. This work provides a reliable tool for monitoring of endogenous HClO in vivo and in vitro, and it has great potential for future research in HClO-related biology and pathology.

HClO是重要的信号传递物质，与生物体的许多疾病和生理活动密切相关，并且在免疫系统中起重要作用。因此，准确，快速地监测生物体内的HClO具有重要意义。但是，体内检测内源HClO仍然是一个挑战。在此，开发了用于快速检测HClO的近红外荧光探针CyClOP。CyClOP具有许多优点，例如响应速度快，选择性好和穿透力强。CyClOP已成功应用于检测活细胞中的内源性HClO。另外，CyClOP首次有效捕获了金黄色葡萄球菌感染和活体小鼠肌肉组织损伤期间免疫系统产生的内源性HClO的波动。这项工作为体内和体外监测内源性HClO提供了可靠的工具，在HClO相关生物学和病理学方面的未来研究中具有巨大的潜力。