Hypoxia in working muscles during exercise may be associated with increased oxidative stress. Inhalation of hyperoxic gas diminishes the hypoxia within working muscles during exercise. Exposure to hyperoxia increases the expression of the antioxidant HSP27. We investigated the effects of acute high-intensity interval exercise (HIE) under hyperoxia on HSP27 levels and oxidative stress responses. Eight male subjects participated in two experiments: 1) normoxic HIE (NHIE) and 2) hyperoxic (60 % oxygen) HIE (HHIE). HIE consisted of four 30-s all-out cycling bouts with 4-min rest between bouts. Levels of serum oxidative stress markers (d-ROMs and LPO), HSP27, BAP, IL-6, and TNF-α significantly increased after both trials. The HIE-induced changes in d-ROMs, LPO, and HSP27 levels were significantly lower in the HHIE trial than in the NHIE trial. These findings suggest that inhaling hyperoxic gas during exercise might diminish oxidative stress induced by all-out HIE.

运动期间工作肌肉的缺氧可能与氧化应激增加有关。吸入高氧气体可减少运动过程中工作肌肉内的缺氧。暴露于高氧会增加抗氧化剂 HSP27 的表达。我们研究了高氧条件下急性高强度间歇运动 (HIE) 对 HSP27 水平和氧化应激反应的影响。八名男性受试者参加了两个实验：1) 常氧 HIE (NHIE) 和 2) 高氧（60% 氧气）HIE (HHIE)。HIE 包括四场 30 秒的全力骑行比赛，每场比赛之间休息 4 分钟。两项试验后，血清氧化应激标志物（d-ROM 和 LPO）、HSP27、BAP、IL-6 和 TNF-α 的水平显着增加。在 HHIE 试验中，HIE 引起的 d-ROM、LPO 和 HSP27 水平的变化显着低于 NHIE 试验。