Abstract In this study, sequential and simultaneous strategies of ultrasonication (ultrasonic bath) were investigated to enhance enzymatic production of N-acetyl glucosamine (GlcNAc) from chitin powder. For the sequential strategy, the ultrasonic caused chitin powder to a visible fleecy structure, and Scanning electron microscopy (SEM) showed the surface of the treated chitin had a fiber-like structure with a diameter of 50 − 200 nm. Moreover, Fourier transform infrared spectroscopy (FTIR), Element analysis (EA), and X-ray diffraction (XRD) revealed that the crystallinity of the chitin decreased with little deacetylation. The simultaneous strategy is a one-pot treatment and enzymatic hydrolysis of chitin. The concentration of GlcNAc was 2.65 g/L for the strategy, which was 1.18- and 5.0-fold higher than the sequential strategy (2.25 g/L) and untreated chitin (0.53 g/L), respectively. In conclusion, this approach provided an efficient and environmentally friendly method for reducing the crystallinity of chitin and enhancing its enzymatic hydrolysis.

中文翻译：

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超声处理对甲壳素酶水解为 N-乙酰氨基葡萄糖的连续和同步策略的影响

摘要 在这项研究中，研究了超声波处理（超声波浴）的顺序和同步策略，以提高从几丁质粉末中酶促生产 N-乙酰氨基葡萄糖 (GlcNAc)。对于顺序策略，超声波使几丁质粉末形成可见的毛状结构，扫描电子显微镜 (SEM) 显示处理后的几丁质表面具有直径为 50 - 200 nm 的纤维状结构。此外，傅里叶变换红外光谱 (FTIR)、元素分析 (EA) 和 X 射线衍射 (XRD) 表明，几丁质的结晶度降低，几乎没有脱乙酰作用。同步策略是一锅处理和几丁质酶水解。该策略的 GlcNAc 浓度为 2.65 g/L，比序贯策略 (2. 25 g/L) 和未处理的几丁质 (0.53 g/L)。总之，该方法为降低几丁质的结晶度并增强其酶促水解提供了一种有效且环保的方法。