Pseudomonas aeruginosa (PA) is an opportunistic pathogen of humans and animals and a common source of nosocomial infections especially of the respiratory tract. Pseudomonas aeruginosa is also a major bacterial disease of poultry and in particular, eggs and newly hatched chicks. In this study, we developed a simple, accurate and rapid molecular detection method using cross priming amplification (CPA) with a nucleic acid test strip to detect P. aeruginosa. The assay efficiently amplified the target gene within 45 min at 62°C only using a simple water bath. The detection limit of the method was 1.18×10² copies μL⁻¹ for plasmid DNA and 4.4 CFU mL⁻¹ for bacteria in pure culture, and was 100 times more sensitive than conventional PCR. We screened 83 clinical samples from yellow-feather broiler breeder chickens and hospitalized/treated dogs and cats using CPA, PCR and traditional culture methods. The positive-sample ratios were 15.3% (13/83) by CPA, 13.3% (11/83) by PCR and 12.1% (10/83) by the culture method. The established CPA method has significant advantages for detecting P. aeruginosa. The method is easy to use and possesses high specificity and sensitivity without the requirements of complicated experimental equipment. The PA-CPA assay is especially fit for outdoor and primary medical units and is an ideal system for the rapid detection and monitoring of P. aeruginosa.

铜绿假单胞菌（PA）是人和动物的机会病原体，是医院尤其是呼吸道感染的常见来源。铜绿假单胞菌也是家禽的主要细菌性疾病，尤其是鸡蛋和新孵出的雏鸡。在这项研究中，我们开发了一种简单，准确和快速的分子检测方法，该方法使用交叉引物扩增（CPA）和核酸试纸来检测铜绿假单胞菌。仅使用简单的水浴，该测定法即可在62°C下45分钟内有效扩增靶基因。该方法的检出限为1.18×10 ^2个拷贝μL ^-1（质粒DNA）和4.4 CFU mL ^-1对于纯培养中的细菌，其灵敏度是常规PCR的100倍。我们使用CPA，PCR和传统培养方法从黄羽肉鸡种鸡和住院/治疗的猫狗中筛选了83个临床样品。CPA的阳性样本比率为15.3％（13/83），PCR的阳性样本比率为13.3％（11/83），培养方法为12.1％（10/83）。建立的CPA方法在检测铜绿假单胞菌方面具有显着优势。该方法简便易行，具有很高的特异性和灵敏度，无需复杂的实验设备。PA-CPA分析特别适合于室外和基层医疗单位，是快速检测和监测铜绿假单胞菌的理想系统。