Experimental Eye Research ( IF 3.4 ) Pub Date : 2020-09-11 , DOI: 10.1016/j.exer.2020.108240 Yuan-Yuan He 1 , Lu Wang 1 , Tao Zhang 1 , Shi-Jun Weng 1 , Jian Lu 2 , Yong-Mei Zhong 1
Aerobic exercise has been shown to play a crucial role in preventing neurological diseases and improving cognitive function. In the present study, we investigated the effect of treadmill training on retinal ganglion cells (RGCs) following optic nerve transection in adult rats. We exercised the rats on a treadmill for 5 d/week (30 min/d at a rate of 9 m/min) or placed control rats on static treadmills. After 3 weeks of exercise, the left optic nerve of each rat was transected. After the surgery, the rat was exercised for another week. The percentages of surviving RGCs in the axotomized eyes of inactive rats were 67% and 39% at 5 and 7 days postaxotomy, respectively. However, exercised rats had significant more RGCs at 5 (74% survival) and 7 days (48% survival) after axotomy. Moreover, retinal brain-derived neurotrophic factor (BDNF) protein levels were significantly upregulated in response to exercise compared with those in the axotomized eyes of inactive rats. Blocking BNDF signaling during exercise by intraperitoneal injections of ANA-12, a BDNF tropomyosin receptor kinase (TrkB) receptor antagonist, reduced the number of RGCs in exercised rats to the level of RGCs in the inactive rats, effectively abolishing the protection of RGCs afforded by exercise. The results suggest that treadmill training effectively rescues RGCs from neurodegeneration following optic nerve transection by upregulating the expression of BDNF.
中文翻译:
有氧运动可延缓视神经损伤后视网膜神经节细胞的死亡。
有氧运动已被证明在预防神经系统疾病和改善认知功能中起着至关重要的作用。在本研究中,我们调查了成年大鼠视神经横断后跑步机训练对视网膜神经节细胞(RGC)的影响。我们在跑步机上以5 d /周(30分钟/天,以9 m / min的速度)锻炼大鼠,或将对照组大鼠放在静态跑步机上。运动3周后,将每只大鼠的左视神经切断。手术后,大鼠再运动一周。在非活动期大鼠切开后第5天和第7天,在无轴索切除的动物的眼睛中存活的RGC百分比分别为67%和39%。然而,运动后的大鼠在轴切术后第5天(存活率74%)和第7天(存活率48%)有更多的RGC。此外,视网膜神经源性神经营养因子(BDNF)蛋白水平显着上调,与不活动大鼠经轴突切除的眼睛相比。通过腹膜内注射BDNF原肌球蛋白受体激酶(TrkB)受体拮抗剂ANA-12来阻断运动过程中的BNDF信号传导,将运动大鼠的RGC数量减少到不活动大鼠的RGC水平,从而有效地消除了RGC对RGC的保护行使。结果表明,跑步机训练可通过上调BDNF的表达有效地将RGC从视神经横断后的神经变性中拯救出来。BDNF原肌球蛋白受体激酶(TrkB)受体拮抗剂可将运动大鼠的RGC数量减少到不活动大鼠的RGC水平,从而有效地消除运动对RGC的保护。结果表明,跑步机训练可通过上调BDNF的表达有效地将RGC从视神经横断后的神经变性中拯救出来。BDNF原肌球蛋白受体激酶(TrkB)受体拮抗剂可将运动大鼠的RGC数量减少到不活动大鼠的RGC水平,从而有效地消除运动对RGC的保护。结果表明,跑步机训练可通过上调BDNF的表达有效地将RGC从视神经横断后的神经变性中拯救出来。