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Silicate solubilizing bacteria UPMSSB7, a potential biocontrol agent against white root rot disease pathogen of rubber tree
Journal of Rubber Research ( IF 1.3 ) Pub Date : 2020-09-11 , DOI: 10.1007/s42464-020-00052-w
Imran Shabbir , Mohd Yusoff Abd Samad , Radziah Othman , Mui-Yun Wong , Zulkefly Sulaiman , Noraini Md Jaafar , Syed Asad Hussain Bukhari

Rigidoporus microporus is a causal agent of white root rot disease of rubber trees. Chemical control is commonly used against this disease but it is expensive, causes environmental pollution and fungal resistance. Silicon is a beneficial element which enhances plant growth and disease resistance but mostly present in insoluble forms. Silicate solubilizing bacteria (SSB) can solubilize the insoluble forms of silicates. This study aimed to screen and evaluate potential SSB with solubilizing activity, plant growth promoting traits and antagonistic activity against R. microporus. Bacteria were isolated from healthy rubber plantation area. Five out of 26 bacterial isolates which showed the ability to solubilize silicate on magnesium trisilicate media were screened in vitro for growth promoting traits and production of indole-3-acetic acid (IAA), siderophores and hydrolytic enzymes. The investigation of antagonistic activity against R. microporus was conducted using dual culture test. The potential SSB isolates were identified by 16S rRNA gene sequencing. The isolate UPMSSB7 showed the highest solubilization of silicate, phosphate (P) and potassium (K) (solubilizing index 4.67, 2.52 and 2.61, respectively). It also showed highest silicate solubilization at 5 and 10 days (9.76 and 11.55 mg L−1, respectively) in liquid assay. It strongly inhibited the growth of R. microporus with percent inhibition of radial growth of 57.24% and produced highest IAA (19.96 µg mL−1). All the isolates produced siderophores. Two isolates, UPMSSB7 and UPMSSB10, were able to secrete cellulase enzyme, but pectinase was produced by UPMSSB7 only. The isolate UPMSSB7 was identified as Enterobacter sp. This isolate was found to be the most potent antagonistic isolate against R. microporus and simultaneously the most efficient one in solubilizing insoluble silicates, as well as P and K.



中文翻译:

硅酸盐增溶细菌UPMSSB7,一种潜在的防治橡胶树白根腐病病原体的生物防治剂

刚毛孔菌是橡胶树白根腐病的病因。化学防治通常用于抵抗这种疾病,但是它很昂贵,会造成环境污染和抗真菌性。硅是有益的元素,可增强植物的生长和抗病性,但大多数以不溶形式存在。硅酸盐增溶细菌(SSB)可以增溶不溶形式的硅酸盐。这项研究旨在筛选和评估具有增溶活性,促进植物生长的性状和对微孔菌的拮抗活性的潜在SSB 。从健康橡胶种植区分离细菌。在体外筛选出能够在三硅酸镁培养基上溶解硅酸盐的26种细菌分离物中的5种,用于体外生长促进性状和吲哚-3-乙酸(IAA),铁载体和水解酶的生产。使用双重培养试验进行了对微孔菌的拮抗活性的研究。通过16S rRNA基因测序鉴定了潜在的SSB分离物。分离物UPMSSB7显示出最高的硅酸盐,磷酸盐(P)和钾(K)增溶性(增溶指数分别为4.67、2.52和2.61)。在液体试验中,在第5天和第10天(分别为9.76和11.55 mg L -1),硅酸盐溶解度最高。它强烈抑制了微孔霉菌对径向生长的抑制百分比为57.24%,并产生最高的IAA(19.96 µg mL -1)。所有分离物均产生铁载体。两种分离物UPMSSB7和UPMSSB10能够分泌纤维素酶,但果胶酶仅由UPMSSB7产生。分离物UPMSSB7被鉴定为肠杆菌属。发现该分离物是针对微孔菌的最有效的拮抗分离物,同时也是增溶不溶性硅酸盐以及P和K的最有效分离物。

更新日期:2020-09-11
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