The strictly anaerobic serum bottles were applied to investigate methane oxidation coupled to chlorate (ClO₃⁻) reduction (MO-CR) without exogenous oxygen. 0.35 mM ClO₃⁻ was consumed within 20 days at the reduction rate of 17.50 μM/d, over three times than that of ClO₄⁻. Chlorite (ClO₂⁻) was not detected throughout the experiment and the mass recovery of Cl⁻ was over 89%. Isotope tracing results showed most of ¹³CH₄ was oxided to CO₂, and the electrons recovery reached to 77.6%. Small amounts of ¹³CH₄ was consumed for DOC production probably through aerobic methane oxidation process, with oxygen generated from disproportionation reaction. In pMMO (key enzyme in aerobic oxidation of methane) inhibition tests, ClO₃⁻ reduction rate was slowed to 7. 0 μmol/d by 2 mM C₂H₂, real-time quantitative PCR also showed the transcript abundance of pMMO and Cld were significantly dropped at the later period of experiment, indicating that the O₂ disproportionated from ClO₂⁻ was utilized to active CH₄. NC10 bacteria Candidatus Methylomirabilis, related closely to oxygenic denitrifiers M. oxyfera, was detected in the system, and got enriched along with chlorate reduction. Several pieces of evidence supported that NC10 bacteria promoted CH₄ oxidation coupled to ClO₃⁻ reduction, these oxygenic denitrifiers may perform ClO₂⁻ disproportionation to produce O₂, and then oxidized methane intracellularly.

在严格厌氧血清瓶施加到调查耦合到氯酸盐甲烷氧化（CLO ₃ ^- ）还原（MO-CR）在没有外源的氧。0.35毫CLO ₃ ^-在17.50μM/ d的减小率在20天内被消耗，在比CLO的三倍₄ ^- 。氯酸钠（CLO ₂ ^- ）没有在整个实验和Cl的质量回收率检测^-超过89％。同位素示踪结果表明，¹³ CH _4的大部分被氧化为CO ₂，电子回收率达到77.6％。少量¹³ CH ₄可能通过需氧甲烷氧化过程消耗了DOC来生产DOC，歧化反应产生了氧气。在单加氧酶（以甲烷的有氧氧化关键酶）抑制试验，CLO ₃ ^-还原速率减慢至7 0微摩尔/ d 2 4.0mm的C ₂ ħ ₂，实时定量PCR也表现出的转录物丰度单加氧酶和卡尔-费舍尔均显著下降在实验的后期，表明将O ₂从CLO歧化₂ ^-被用于有源CH ₄。NC10细菌甲基念珠菌在系统中检测到与氧反硝化菌氧化羟色菌密切相关的，并随着氯酸盐的还原而富集。若干证据件支持该NC10细菌促进CH ₄氧化耦合至C10 ₃ ^-还原，这些产氧反硝化可以执行CLO ₂ ^-歧化产生Ò ₂，然后氧化甲烷细胞内。