Staphylococcus aureus and Staphylococcus lugdunensis are often associated with pathogenic biofilms ranging from superficial mucosal to life-threatening systemic infections. Recent studies have reported that chelerythrine (CHE) displays antimicrobial activities against a few microorganisms, but its effects on dual-species biofilms of S. aureus and S. lugdunensis have never been reported. The purpose of this study was to investigate how dual-species biofilms of S. aureus and S. lugdunensis respond when challenged with CHE. Minimum inhibitory concentration (MIC) of CHE against planktic cells in dual-species culture was 8 μg/mL. CHE also suppressed dual-species biofilm formation at minimal biofilm inhibitory concentration (MBIC₉₀, 4 μg/mL). Further, confocal laser scanning microscope (CLSM) using five fluorescent dyes revealed the dose-dependent reduction of the levels of three key biofilm matrix components, and reduced tolerance to gatifloxacin, of biofilms exposed to CHE. Moreover, CHE efficiently eradicated preformed dual-species biofilms at minimal biofilm eradication concentration (MBEC, 256 μg/mL). Hence, CHE has the potential to address biofilm infections of clinical course and other biofilm-related diseases caused by S. aureus and S. lugdunensis.

金黄色葡萄球菌和路登葡萄球菌通常与从浅表黏膜到危及生命的全身感染的病原生物膜有关。最近的研究报道，白屈菜红碱 (CHE) 对少数微生物具有抗菌活性，但从未报道过其对金黄色葡萄球菌和S. lugdunensis双物种生物膜的影响。本研究的目的是研究S. aureus和S. lugdunensis 的双种生物膜如何当受到 CHE 挑战时做出回应。CHE 在双物种培养中对浮游细胞的最小抑制浓度 (MIC) 为 8 μg/mL。CHE 还在最小生物膜抑制浓度 (MBIC ₉₀ , 4 μg/mL) 下抑制双物种生物膜形成。此外，使用五种荧光染料的共聚焦激光扫描显微镜 (CLSM) 揭示了暴露于 CHE 的生物膜的三种关键生物膜基质成分水平的剂量依赖性降低，以及对加替沙星的耐受性降低。此外，CHE 以最小的生物膜根除浓度（MBEC，256 μg/mL）有效地根除预先形成的双物种生物膜。因此，CHE有可能解决由金黄色葡萄球菌和路顿葡萄球菌引起的临床病程和其他生物膜相关疾病的生物膜感染.