The current study presents a method based on Optical Coherence Tomography (OCT) for non-destructive, real-time analysis and portrayal of immobilization efficacy for lipase on a natural matrix namely, eggshell. Subsequently, qualitative biochemical reaction kinetics of immobilized lipase was also studied. Successful immobilization of lipase on eggshell was confirmed by the presence of a clear peak in ‘A’ scan of OCT image. From immobilization kinetics it is clearly observed that the thickness of the highest peak of the A-scan increases significantly and peak intensity saturated after 90 min of incubation. Hydrolysis of oil using immobilized lipase indicated that the release of free fatty acids increased up to 8 h during reaction and the result was in accordance with the ‘B’ scan data of the OCT system. Changes in scattering coefficient-based analysis were performed with respect to incubation time to showcase the immobilization process and hydrolysis reaction of lipase. Scanning electron microscope analysis with smoother surface indicated presence of lipase on eggshell matrices, with no further change after oil hydrolysis.

目前的研究提出了一种基于光学相干断层扫描 (OCT) 的方法，用于无损、实时分析和描绘天然基质上脂肪酶的固定功效，即蛋壳。随后，还研究了固定化脂肪酶的定性生化反应动力学。脂肪酶在蛋壳上的成功固定通过 OCT 图像“A”扫描中清晰峰的存在证实。从固定动力学可以清楚地观察到，A 扫描最高峰的厚度显着增加，并且在孵育 90 分钟后峰值强度达到饱和。使用固定化脂肪酶水解油表明，在反应过程中游离脂肪酸的释放增加了 8 小时，结果与 OCT 系统的“B”扫描数据一致。对孵育时间进行基于散射系数的分析的变化，以展示脂肪酶的固定过程和水解反应。具有更光滑表面的扫描电子显微镜分析表明蛋壳基质上存在脂肪酶，油水解后没有进一步变化。