This study reveals out detoxifying and antioxidant enzyme response of Gammarus pulex exposed/polluted to chlorpyrifos-ethyl insecticide before and after biodegradation/bioremediation by Methylobacterium radiotolerans and Microbacterium arthrosphaerae. Cytochrome P450 1A1, glutathione S-transferase, catalase, and superoxide dismutase activities in G. pulex exposed to chlorpyrifos-ethyl before and after bioremediation/biodegradation by these two bacteria during 24 and 96 h tested by using commercial ELISA kits. The activity of catalase enzyme was decreased depending on chlorpyrifos-ethyl before and after bioremediation/biodegradation the enzyme activity was increased repeatedly. Superoxide dismutase activity level increased after chlorpyrifos-ethyl exposure in 96 h (p > 0.05). Following bioremediation, superoxide dismutase enzyme activity decreased again during 24 h (p > 0.05) and increased during 96 h (p < 0.05). Statistical differences were not found in cytochrome P450 1A1 enzyme activity before and after the process (p > 0.05). No significant differences were determined during the activity of glutathione S-transferase in 24 h (p > 0.05). The activities of glutathione S-transferase were increased after exposure of chlorpyrifos-ethyl during 96 h. After bioremediation; the activity of glutathione S-transferase increased even more (p < 0.05). The results determined that activities of G. pulex at superoxide dismutase, catalase, and glutathione S-transferase are common biomarkers for revealing out the efficiency of bioremediation of chlorpyrifos-ethyl with these two types of soil bacteria. Graphical abstract.

中文翻译：

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利用一些生化生物标记物的响应，评估耐甲基甲烷杆菌和节肢微细菌对毒死rif的修复效率。

这项研究揭示了暴露/污染了毒死ethyl-乙基杀虫剂的γ-粉虱的解毒和抗氧化酶反应，之前和之后由耐甲基甲基细菌和节肢微细菌进行生物降解/生物修复。使用市售ELISA试剂盒测试了在这两种细菌进行生物修复/生物降解之前和之后暴露于毒死-乙基的G.pulex中的细胞色素P450 1A1，谷胱甘肽S-转移酶，过氧化氢酶和超氧化物歧化酶活性。在生物修复/生物降解之前和之后，取决于毒死-的乙基，过氧化氢酶的活性降低，酶活性反复增加。毒死rif乙基暴露96小时后，超氧化物歧化酶活性水平升高（p> 0.05）。经过生物修复后，超氧化物歧化酶活性在24 h内再次降低（p> 0.05），在96 h内再次升高（p <0.05）。在该过程之前和之后，细胞色素P450 1A1酶活性均未发现统计学差异（p> 0.05）。在24小时内谷胱甘肽S-转移酶活性期间未发现显着差异（p> 0.05）。毒死rif乙基暴露96小时后，谷胱甘肽S-转移酶的活性增加。生物修复后；谷胱甘肽S-转移酶的活性增加更多（p <0.05）。该结果确定了葛根芽孢杆菌对超氧化物歧化酶，过氧化氢酶和谷胱甘肽S-转移酶的活性是常见的生物标志物，用于揭示用这两种土壤细菌对毒死bio进行生物修复的效率。图形概要。