mRNAs play key roles in regulating diverse cellular functions. In many cases, mRNAs exhibit distinct intracellular localizations that are necessary for the spatiotemporal control of protein expression in cells. Therefore, imaging the localization and dynamics of these mRNAs is crucial for understanding diverse aspects of cellular function. In this review, we summarize how mRNA imaging can be achieved using tethered fluorescent proteins and fluorogenic aptamers. We discuss 'fluorogenic proteins' and describe how these recently developed RNA-regulated fluorescent proteins simplify mRNA imaging experiments.

中文翻译：

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使用荧光蛋白对活细胞中的 mRNA 运输进行成像。

mRNA 在调节多种细胞功能方面发挥着关键作用。在许多情况下，mRNA 表现出不同的细胞内定位，这是细胞中蛋白质表达的时空控制所必需的。因此，对这些 mRNA 的定位和动力学进行成像对于理解细胞功能的各个方面至关重要。在这篇综述中，我们总结了如何使用系留荧光蛋白和荧光适体实现 mRNA 成像。我们讨论了“荧光蛋白”，并描述了这些最近开发的 RNA 调节荧光蛋白如何简化 mRNA 成像实验。