Clostridium tyrobutyricum is a promising microbial host for the anaerobic production of bio-based chemicals, especially butyric acid. At the same time, it also has great potential as a probiotic for the production of short-chain fatty acids in the intestines. However, due to the insufficient knowledge of the genetic characteristics of this organism, there has been little progress in its genetic engineering to date. Here, we present the complete genome sequence of C. tyrobutyricum L319, which consists of a circular chromosome and a plasmid with a G + C content of 31.69%, encompassing approximately 3.09 Mb with 3052 protein-coding genes. Functional gene annotation revealed better results than previous studies based on KEGG pathway classification. Furthermore, we obtained detailed functional characterization of 93 genes previously annotated as putative proteins. Genomic analysis revealed that this organism contains multiple genes encoding enzymes involved in the CRISPR-Cas systems, substrate utilization, isopeptide and ester bonds, transcriptional regulation, and oxidative stress. The safety evaluation at genetic level indicated that this organism does not possess transferable resistance genes, invasive defensive pathogenicity factors, or harmful enzymes. The genome sequence data analyzed in this study will be available for further research and will facilitate the further understanding and development of potential applications of C. tyrobutyricum.

中文翻译：

---

酪丁酸梭菌的基因组序列分析，一种用于人类健康和工业应用的有前景的微生物宿主

酪丁酸梭菌是一种很有前景的微生物宿主，可用于厌氧生产生物基化学品，尤其是丁酸。同时，它作为一种益生菌在肠道中产生短链脂肪酸也具有很大的潜力。然而，由于对该生物的遗传特性认识不足，迄今为止其基因工程进展甚微。在这里，我们展示了酪丁酸梭菌 L319 的完整基因组序列，它由一个环状染色体和一个 G+C 含量为 31.69% 的质粒组成，包含大约 3.09 Mb 和 3052 个蛋白质编码基因。功能基因注释显示出比以前基于 KEGG 通路分类的研究更好的结果。此外，我们获得了先前注释为推定蛋白质的 93 个基因的详细功能表征。基因组分析显示，该生物体包含多个基因，这些基因编码参与 CRISPR-Cas 系统、底物利用、异肽和酯键、转录调控和氧化应激的酶。基因水平的安全性评价表明，该生物不具有可转移的抗性基因、侵入性防御致病因素或有害酶。本研究中分析的基因组序列数据将可用于进一步研究，并将促进对酪丁酸梭菌潜在应用的进一步理解和开发。异肽键和酯键、转录调节和氧化应激。基因水平的安全性评价表明，该生物不具有可转移的抗性基因、侵入性防御致病因素或有害酶。本研究中分析的基因组序列数据将可用于进一步研究，并将促进对酪丁酸梭菌潜在应用的进一步理解和开发。异肽键和酯键、转录调节和氧化应激。基因水平的安全性评价表明，该生物不具有可转移的抗性基因、侵入性防御致病因素或有害酶。本研究中分析的基因组序列数据将可用于进一步研究，并将促进对酪丁酸梭菌潜在应用的进一步理解和开发。