Imaging mass spectrometry has become a mature molecular mapping technology that is used for molecular discovery in many medical and biological systems. While powerful by itself, imaging mass spectrometry can be complemented by the addition of other orthogonal, chemically informative imaging technologies to maximize the information gained from a single experiment and enable deeper understanding of biological processes. Within this review, we describe MALDI, SIMS, and DESI imaging mass spectrometric technologies and how these have been integrated with other analytical modalities such as microscopy, transcriptomics, spectroscopy, and electrochemistry in a field termed multimodal imaging. We explore the future of this field and discuss forthcoming developments that will bring new insights to help unravel the molecular complexities of biological systems, from single cells to functional tissue structures and organs.

中文翻译：

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多模式成像质谱：生物学和医学中的下一代分子图谱。

成像质谱已成为一种成熟的分子作图技术，用于许多医学和生物系统中的分子发现。虽然成像质谱本身功能强大，但可以通过添加其他正交的化学信息成像技术来补充，以最大限度地从单个实验中获得信息并加深对生物过程的理解。在这篇综述中，我们描述了 MALDI、SIMS 和 DESI 成像质谱技术，以及这些技术如何与称为多模式成像的领域中的其他分析模式（如显微镜、转录组学、光谱学和电化学）相结合。