Inhalation of silicon dioxide (SD) results in pulmonary inflammatory responses and fibrosis. Isorhynchophylline (Isorhy) is the main alkaloid in the traditional Chinese herb Tripterygium wilfordii, which is reported to have anti-inflammatory activities in the nervous system. However, the effects of Isorhy on SD-induced pulmonary inflammation and fibrosis in mice are unknown. Male mice were exposed to a single dose of SD (2.5 mg/kg, intranasal inoculation) to induce pulmonary fibrosis (PF). The mice were woken up and immediately treated with Isorhy (20 mg/kg, intraperitoneal injection) for 14 or 42 days. The effects of Isorhy on inflammatory responses and lung fibrosis induced by SD were then investigated. After the 14-day treatment, there was a significant reduction in inflammatory cell infiltration in the lungs of mice, with reduced recruitment of inflammatory cells to the lungs. The concentration of pro-inflammatory factors in the bronchoalveolar lavage fluid was reduced, which alleviated inflammatory injury in the lung tissue. After the 42-day treatment, Isorhy alleviated inflammation and inhibited the release of fibrogenic factors in mice with PF. Isorhy also significantly reduced collagen deposition in the lung tissues of mice. Isorhy has the ability to reduce inflammatory responses and fibrosis associated with SD-induced acute lung injury.

中文翻译：

---

异去氧茶碱对小鼠二氧化硅引起的肺损伤的保护作用。

吸入二氧化硅（SD）会导致肺部炎症反应和纤维化。异叶胆碱（Isorhy）是传统中草药雷公藤的主要生物碱，据报道其在神经系统中具有抗炎活性。但是，Isorhy对SD诱发的小鼠肺部炎症和纤维化的作用尚不清楚。将雄性小鼠暴露于单剂量的SD（2.5 mg / kg，鼻内接种）以诱发肺纤维化（PF）。唤醒小鼠并立即用Isorhy（20mg / kg，腹膜内注射）治疗14或42天。然后研究了Isorhy对SD引起的炎症反应和肺纤维化的影响。经过14天的治疗，小鼠肺部炎症细胞浸润明显减少，减少炎症细胞向肺部的募集。降低了支气管肺泡灌洗液中促炎因子的浓度，减轻了肺组织的炎症损伤。经过42天的治疗，Isorhy减轻了PF小鼠的炎症并抑制了纤维化因子的释放。Isorhy还显着减少了小鼠肺组织中的胶原蛋白沉积。Isorhy具有减少与SD诱发的急性肺损伤相关的炎症反应和纤维化的能力。Isorhy还显着减少了小鼠肺组织中的胶原蛋白沉积。Isorhy具有减少与SD诱发的急性肺损伤相关的炎症反应和纤维化的能力。Isorhy还显着减少了小鼠肺组织中的胶原蛋白沉积。Isorhy具有减少与SD诱发的急性肺损伤相关的炎症反应和纤维化的能力。