Plant tissue culture techniques have been used to propagate horticultural crops at a commercial scale for more than three decades. However, due to the high cost it is generally only used for high value crops. To increase production efficiency and make micropropagation viable for a wider range of species, new approaches to address key steps of the process with high labor inputs need to be evaluated. For this study, a two‐piece scaffold system was designed, prototyped using 3D printing, and tested to physically hold plants upright thereby facilitating liquid based rooting. This system was evaluated with Malus domestica, Betula lenta, and Musa sp. using static liquid culture as well as rocker based temporary immersion system and compared to rooting in semi‐solid based medium as is commonly practiced. Significantly, earlier rooting was observed in all three species in liquid when compared to semi‐solid culture system, and plants cultured in liquid on the rocker generally performed better than those in static liquid. In addition to quicker, more uniform rooting, reducing labor requirements, and preventing root damage. This newly designed system is simple, easy to use, will help to improve efficiency, and reduce the cost of micropropagation.

中文翻译：

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使用两件式支架系统改进液体培养中的体外生根

植物组织培养技术已被用于以商业规模繁殖园艺作物超过三十年。但由于成本高，一般只用于高价值作物。为了提高生产效率并使微繁殖适用于更广泛的物种，需要评估解决高劳动力投入的过程关键步骤的新方法。在这项研究中，设计了一个两件式支架系统，使用 3D 打印进行原型设计，并进行测试以将植物物理保持直立，从而促进液体生根。该系统用 Malusdomestica、Betula lenta 和 Musa sp. 进行了评估。使用静态液体培养以及基于摇杆的临时浸入系统，并与通常采用的半固体培养基进行比较。显着地，与半固体培养系统相比，在液体中的所有三个物种都观察到更早的生根，并且在摇杆上的液体中培养的植物通常比静态液体中的植物表现更好。除了更快、更均匀地生根，减少劳动力需求并防止根部损坏。这种新设计的系统简单、易于使用，将有助于提高效率，并降低微繁殖的成本。