ABSTRACT

This study investigated the effects of five cephalosporin antibiotics (ceftazidime, ceftriaxone, cefdinir, cefixime and cefepime) on performance and bacterial community structure in bio-electrochemical systems (BES) and sequencing batch biofilm reactor (SBBR). The results showed that the external electric field had no significant effect on the removal of COD and ammonia nitrogen in water. The removal rates of five antibiotics in BES increased by 28.5%, 20.0%, 9.1%, 21.0%, and 11.5%, respectively. High-through sequencing showed that microbial membrane-growing process increased species diversity, and antibiotics had a significant inhibitory effect on the initial biofilm of the reactor. As time progressed, the inhibitory effect was weakened, and the microorganism were tolerated and re-enriched. The increase in the type and concentration of antibiotics and the applied electric field had a significant effect on the microorganisms in the reactor. The dominant microorganisms for antibiotic removal in the SBBR were Luteococcus, Cloacibacterium, Dysgonomonas, and Ottowia. The dominant bacteria in the BES were Ottowia and Tahibacte. The abundance of these strains increased significantly during antibiotic acclimation. The abundance of Ottowia, Tahibacter, and Nakamurella were significantly higher than SBBR. Thus the BES system had a good antibiotic degradation effect. The BES can effectively treat simulated domestic sewage containing multiple antibiotics, laying a theoretical foundation for the actual wastewater treatment.

摘要

本研究调查了五种头孢菌素类抗生素（头孢他啶、头孢曲松、头孢地尼、头孢克肟和头孢吡肟）对生物电化学系统 (BES) 和序批式生物膜反应器 (SBBR) 的性能和细菌群落结构的影响。结果表明，外加电场对水中COD和氨氮的去除效果不显着。BES中五种抗生素的去除率分别提高了28.5%、20.0%、9.1%、21.0%和11.5%。高通量测序表明，微生物膜生长过程增加了物种多样性，抗生素对反应器的初始生物膜有显着抑制作用。随着时间的推移，抑制作用减弱，微生物被耐受并重新富集。抗生素种类和浓度的增加以及施加的电场对反应器中的微生物有显着影响。SBBR 中抗生素去除的主要微生物是Luteococcus、Cloacibacterium、Dysgonomonas和Ottowia。BES中的优势菌是Ottowia和Tahibacte。在抗生素驯化过程中，这些菌株的丰度显着增加。Ottowia、Tahibacter和Nakamurella的丰度显着高于SBBR。因此BES系统具有良好的抗生素降解效果。BES可以有效处理含有多种抗生素的模拟生活污水，为实际废水处理奠定理论基础。