Two sets of mononuclear N, O-chelate complexes, 1a [Co(L1)2(H2O)2], 1b [Ni(L1)2] and 1c [Cu(L1)2], and 2a [Co(L2)2(H2O)2], 2b [Ni(L2)2] and 2c [Cu(L2)2], from respective Schiff base ligands, HL1: 2-((E)-(6-(trifluoromethoxy)phenylimino)methyl)-5-methylphenol, C15H12F3NO2) and HL2: 2-((E)-(4-(trifluoromethoxy)phenylimino)methyl)-4,6-dichlorophenol, C14H8Cl2F3NO2) have been synthesized and well-characterized by analytical and various spectroscopic techniques like mass spectrometry, FT-IR, UV-visible, magnetic susceptibility, ESR and thermogravimetric studies. The geometries are proposed as octahedral for complexes 1a and 2a, whereas square planar for complexes 1b, 1c, and 2b, 2c. DNA binding study was investigated against calf thymus DNA for synthesized complexes comparing with parent ligands and resulted in intercalation mode of binding which was further supported by fluorescence quenching and viscometric titrations. Nuclease efficacy was also investigated against supercoiled pBR322 DNA and a good cleavage pattern was shown by metal complexes. The complexes were found to possess good antimicrobial activity compared to free ligands against bacterial species, Bacillus amyloliquefaciens and Escherichia coli as well as fungal species, Macrophomina phaseolina and Sclerotium rolfsii.

中文翻译：

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对源自 N、O 供体希夫碱的单核 Co(II)、Ni(II) 和 Cu(II) 配合物的 DNA 结合、切割和抗菌特性的研究。

两组单核 N、O-螯合物，1a [Co(L1)2(H2O)2]、1b [Ni(L1)2] 和 1c [Cu(L1)2]，以及 2a [Co(L2)2 (H2O)2]、2b [Ni(L2)2] 和 2c [Cu(L2)2]，来自各自的席夫碱配体，HL1：2-((E)-(6-(三氟甲氧基)苯基亚氨基)甲基)- 5-甲基苯酚、C15H12F3NO2) 和 HL2：2-((E)-(4-(三氟甲氧基)苯基亚氨基)甲基)-4,6-二氯苯酚、C14H8Cl2F3NO2) 已被合成并通过分析和各种光谱技术（如质谱）进行了充分表征光谱、FT-IR、UV-可见光、磁化率、ESR 和热重研究。配合物 1a 和 2a 的几何形状被提议为八面体，而配合物 1b、1c 和 2b、2c 的几何形状为方形平面。DNA结合研究针对合成的复合物与亲本配体进行了针对小牛胸腺DNA的研究，并导致结合的嵌入模式，荧光猝灭和粘度滴定进一步支持该模式。还研究了核酸酶对超螺旋 pBR322 DNA 的功效，金属配合物显示出良好的切割模式。发现与针对细菌物种、解淀粉芽孢杆菌和大肠杆菌以及真菌物种、Macrophomina phaseolina 和 Sclerotium rolfsii 的游离配体相比，该复合物具有良好的抗菌活性。