To investigate the impact of total flavonoids of Epimedium (TFE) on the development of follicles of laying hens, 3 types of follicles including primary, prehierarchical, and preovulatory follicles were selected to obtain the follicular granulosa cells cultured in vitro. First, extraction of TFE was conducted by alcohol-soluble and ultrasonic methods. The effects of TFE on activity and proliferation of follicular granulosa cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and measuring the expression of proliferating cell nuclear antigen mRNA through real-time quantitative polymerase chain reaction, and the expression of the follicle-stimulating hormone receptor, luteinizing hormone receptor, steroidogenic acute regulatory protein, and cytochrome P450 family 11 subfamily A member 1 mRNA was detected to study the functions of TFE affecting the differentiation and hormone secretion by granulosa cells. The results showed that TFE significantly improved the proliferation of 3 types of granulosa cells and promoted the differentiation of granulosa cells and accelerated the conversion of primary follicles to prehierarchical follicles. Total flavonoids of Epimedium played an important role in promoting progesterone secretion by prehierarchical and preovulatory granulosa cells. The results indicated that TFE could promote proliferation and differentiation of follicular granulosa cells and improve hormone secretion and follicle development, which provided reference data for TFE used as a feed additive or safe Chinese veterinary medicine to promote the laying rate.

中文翻译：

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淫羊med总黄酮对蛋鸡颗粒细胞发育的影响。

为了研究淫羊total总黄酮（TFE）对蛋鸡卵泡发育的影响，选择了三种类型的卵泡，包括初级卵泡，初级卵泡和排卵前卵泡，以获得体外培养的卵泡颗粒细胞。首先，通过醇溶性和超声方法进行TFE的提取。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物测定并通过实时检测增殖细胞核抗原mRNA的表达来检测TFE对滤泡性颗粒细胞活性和增殖的影响定量聚合酶链反应，促卵泡激素受体，黄体生成激素受体，类固醇生成的急性调节蛋白的表达，检测细胞色素P450家族11亚家族的成员1 mRNA，以研究TFE对粒细胞分化和激素分泌的影响。结果表明，TFE显着改善了3种类型的颗粒细胞的增殖，并促进了颗粒细胞的分化，并促进了初级卵泡向分级前卵泡的转化。淫羊med总黄酮在促进层前和排卵前的颗粒细胞促进孕酮分泌中起重要作用。结果表明，TFE可以促进卵泡颗粒细胞的增殖和分化，改善荷尔蒙分泌和卵泡发育，为TFE作为饲料添加剂或安全的中兽药提高产蛋率提供参考数据。