In the present work, ethyl acetate extracts, consisting of non-volatile compounds, from the culture of endophytic fungi isolated from coffee plants, Induratia coffeana and Induratia yucatanensis, were prospected in enzyme modulation tests that act in human hemostasis. Dry extracts of the fungi were diluted in dimethyl sulfoxide p.a. 99.9% (DMSO), and then tested. Bothrops atrox venom was used as an enzyme source and tool to induce the activities. Prior to the evaluation of the activities, incubations of the extracts with the venom were performed in the proportions 1: 0.01, 1: 0.25, 1: 0.5, and 1: 1 (venom: extract; mass: mass). The extracts of all fungi promoted a significant increase in the clotting time induced by the venom, which was even longer when the extracts were previously incubated with the citrated plasma. The activity of phospholipases A2 did not significantly change when evaluated in the presence of fungal extracts. However, the evaluated extracts inhibited proteases by 73% and 30% in the thrombolytic and caseinolytic tests, respectively. In addition, the extracts did not induce cytotoxicity on human erythrocytes when evaluated in the absence of the venom. Thus, it is possible to suggest the presence of specific interactions between molecules present in extracts of Induratia spp. and venom proteases, highlighting non-volatile metabolites as promising sources of compounds of medical and scientific interest.

中文翻译：

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来自 Induratia spp 的酶调节剂。

在目前的工作中，从咖啡植物 Induratia coffeana 和 Induratia yucatanensis 中分离出的内生真菌培养物中，由非挥发性化合物组成的乙酸乙酯提取物被用于人类止血的酶调节试验。真菌的干提取物在二甲基亚砜 pa 99.9% (DMSO) 中稀释，然后进行测试。Bothrops atrox 毒液用作酶源和诱导活性的工具。在评估活性之前，提取物与毒液的孵育按 1:0.01、1:0.25、1:0.5 和 1:1（毒液：提取物；质量：质量）的比例进行。所有真菌的提取物都促进了毒液诱导的凝血时间的显着增加，当提取物之前与柠檬酸化血浆一起孵育时，凝血时间甚至更长。在真菌提取物的存在下进行评估时，磷脂酶 A2 的活性没有显着变化。然而，评估的提取物在溶栓和酪蛋白溶解试验中分别抑制了 73% 和 30% 的蛋白酶。此外，在没有毒液的情况下评估时，提取物不会对人红细胞产生细胞毒性。因此，有可能表明 Induratia spp. 提取物中存在的分子之间存在特定的相互作用。和毒液蛋白酶，强调非挥发性代谢物是具有医学和科学意义的化合物的有希望的来源。当在没有毒液的情况下评估时，提取物不会对人红细胞产生细胞毒性。因此，有可能表明 Induratia spp. 提取物中存在的分子之间存在特定的相互作用。和毒液蛋白酶，强调非挥发性代谢物是具有医学和科学意义的化合物的有希望的来源。当在没有毒液的情况下评估时，提取物不会对人红细胞产生细胞毒性。因此，有可能表明 Induratia spp. 提取物中存在的分子之间存在特定的相互作用。和毒液蛋白酶，强调非挥发性代谢物是具有医学和科学意义的化合物的有希望的来源。