Vessel damage is a general pathological process in many neurodegenerative disorders, as well as spinal cord injury, stroke, or trauma. Biomaterials can present novel tools to repair and regenerate damaged vessels. The aim of the present study is to test collagen hydrogels loaded with different angiogenic factors to study vessel repair in organotypic brain slice cultures. In the experimental set up I, we made a cut on the organotypic brain slice and tested re-growth of laminin + vessels. In the experimental set up II, we cultured two half brain slices with a gap with a collagen hydrogel placed in between to study endothelial cell migration. In the experimental set up I, we showed that the number of vessels crossing the cut was tendencially increased with the addition of fibroblast growth factor-2 (FGF-2), vascular endothelial growth factor, or platelet-derived growth factor-BB compared to the control group. In the experimental set up II, we demonstrated that a collagen hydrogel loaded with FGF-2 resulted in a significantly increased number of migrated laminin + cells in the gap between the slices compared to the control hydrogel. Co-administration of several growth factors did not further potentiate the effects. Taken together, we show that organotypic brain slices are good models to study brain vessels and FGF-2 is a potent angiogenic factor for endothelial cell proliferation and migration. Our results provide evidence that the collagen hydrogels can be used as an extracellular matrix for the vascular endothelial cells.

血管损伤是许多神经退行性疾病以及脊髓损伤，中风或外伤的一般病理过程。生物材料可以提供新颖的工具来修复和再生受损的血管。本研究的目的是测试载有不同血管生成因子的胶原蛋白水凝胶，以研究器官型脑切片培养物中的血管修复。在实验设置I中，我们切开了器官型脑切片，并测试了层粘连蛋白+血管的重新生长。在实验设置II中，我们培养了两个半脑片，并在它们之间留有间隙，并在其中放置了胶原蛋白水凝胶，以研究内皮细胞的迁移。在实验设置I中，我们显示，通过添加成纤维细胞生长因子2（FGF-2），血管内皮生长因子，跨过切口的血管数量呈趋势性增加，或血小板衍生的生长因子BB与对照组相比。在实验设置II中，我们证明了与对照水凝胶相比，载有FGF-2的胶原蛋白水凝胶在切片之间的间隙中导致迁移的层粘连蛋白+细胞数量显着增加。几种生长因子的共同给药不能进一步增强这种作用。综上所述，我们显示器官型脑切片是研究脑血管的良好模型，而FGF-2是内皮细胞增殖和迁移的有效血管生成因子。我们的结果提供了证据，胶原水凝胶可以用作血管内皮细胞的细胞外基质。我们证明，与对照水凝胶相比，载有FGF-2的胶原水凝胶在切片之间的间隙中导致迁移的层粘连蛋白+细胞数量显着增加。几种生长因子的共同给药不能进一步增强这种作用。综上所述，我们显示器官型脑切片是研究脑血管的良好模型，而FGF-2是内皮细胞增殖和迁移的有效血管生成因子。我们的结果提供了证据，胶原水凝胶可以用作血管内皮细胞的细胞外基质。我们证明，与对照水凝胶相比，载有FGF-2的胶原水凝胶在切片之间的间隙中导致迁移的层粘连蛋白+细胞数量显着增加。几种生长因子的共同给药不能进一步增强这种作用。综上所述，我们显示器官型脑切片是研究脑血管的良好模型，而FGF-2是内皮细胞增殖和迁移的有效血管生成因子。我们的结果提供了证据，胶原水凝胶可以用作血管内皮细胞的细胞外基质。我们显示器官型脑切片是研究脑血管的良好模型，而FGF-2是内皮细胞增殖和迁移的有效血管生成因子。我们的结果提供了证据，胶原水凝胶可以用作血管内皮细胞的细胞外基质。我们显示器官型脑切片是研究脑血管的良好模型，而FGF-2是内皮细胞增殖和迁移的有效血管生成因子。我们的结果提供了证据，胶原水凝胶可以用作血管内皮细胞的细胞外基质。