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Identification, Characterization and Evaluation of Multifaceted Traits of Plant Growth Promoting Rhizobacteria from Soil for Sustainable Approach to Agriculture
Current Microbiology ( IF 2.6 ) Pub Date : 2020-08-28 , DOI: 10.1007/s00284-020-02165-2
Teg Bahadur Singh 1 , Vikram Sahai 2 , Deepika Goyal 1 , Mrinalini Prasad 1 , Arti Yadav 1 , Preksha Shrivastav 1 , Akbar Ali 1 , Prem Kumar Dantu 1
Affiliation  

This study assessed potential of plant growth promoting rhizobacteria (PGPR) found in 34 soil samples collected from Sirmaur, Himachal Pradesh. Out of 238 rhizobacterial isolates, 48 rhizobacterial isolates exhibited multiple PGP (plant growth promoting) traits. Out of the 48 isolates, nine isolates exhibiting most promising PGP traits were evaluated. CSRS12 isolate showed maximum solubilization of phosphate and potassium up to 530.71 and 30.44 mg l-1, respectively. Maximum zinc solubilizing efficiency (ZSE) was also observed in case of isolate CSRS12. The maximum IAA production was observed by isolate PPRS17 with 37.34 mg l-1 followed by PCRS24 with 34.44 mg l-1 after 120 h. Maximum siderophore unit production was observed upto 92.29% by isolate CSRS12 followed by 65.54% with isolate TA1PS. The selected PGPR isolates were identified through 16S rDNA sequencing. The identified PGPRs were Burkholderia arboris (isolate CSRS12), Pseudomonas aeruginosa (isolate PPRS17) and Acinetobacter baumannii (isolate TA1PS). B. arboris CSRS12 isolate showed multiple PGP traits as mineral solubilization of phosphate, potassium and zinc, production of siderophore and ammonia. Among all three PGPR treatment, B. arboris CSRS12 isolate showed significant increment in lateral root number, root and shoot length, fresh and dry weight of root and shoot of mung bean (Vigna radiata) in pot experiments. The results showed that CSRS12 isolate could be used for exploitation as bio-inoculant, which can facilitate better productivity and ecological dynamics for both domesticated crops as well as wild varieties.

中文翻译:

土壤中促进植物生长的根际细菌的多方面性状的鉴定、表征和评估以实现可持续农业方法

本研究评估了从喜马偕尔邦西尔毛尔收集的 34 个土壤样品中发现的植物生长促进根际细菌 (PGPR) 的潜力。在 238 个根际细菌分离株中,48 个根际细菌分离株表现出多种 PGP(植物生长促进)性状。在 48 个分离株中,评估了 9 个表现出最有希望的 PGP 性状的分离株。CSRS12 分离株显示出磷酸盐和钾的最大溶解度,分别高达 530.71 和 30.44 mg l-1。在分离CSRS12的情况下也观察到最大锌溶解效率(ZSE)。120 小时后,分离株 PPRS17 的 IAA 产量最高,为 37.34 mg l-1,随后 PCRS24 为 34.44 mg l-1。分离株CSRS12观察到的最大铁载体单位产量高达92.29%,其次是分离株TA1PS的65.54%。选定的 PGPR 分离株通过 16S rDNA 测序进行鉴定。鉴定出的 PGPR 是伯克霍尔德菌(分离 CSRS12)、铜绿假单胞菌(分离 PPRS17)和鲍曼不动杆菌(分离 TA1PS)。B. arboris CSRS12 分离株显示出多种 PGP 特性,如磷酸盐、钾和锌的矿物质溶解、铁载体和氨的产生。在所有三种 PGPR 处理中,B. arboris CSRS12 分离株在盆栽试验中显示出绿豆(Vigna radiata)的侧根数、根和枝条长度、根和枝条鲜重和干重的显着增加。结果表明,CSRS12 分离株可用作生物接种剂,可促进驯化作物和野生品种提高生产力和生态动态。鉴定出的 PGPR 是伯克霍尔德菌(分离 CSRS12)、铜绿假单胞菌(分离 PPRS17)和鲍曼不动杆菌(分离 TA1PS)。B. arboris CSRS12 分离株显示出多种 PGP 特性,如磷酸盐、钾和锌的矿物质溶解、铁载体和氨的产生。在所有三种 PGPR 处理中,B. arboris CSRS12 分离株在盆栽试验中显示出绿豆(Vigna radiata)的侧根数、根和枝条长度、根和枝条鲜重和干重的显着增加。结果表明,CSRS12 分离株可用作生物接种剂,可促进驯化作物和野生品种提高生产力和生态动态。鉴定出的 PGPR 是伯克霍尔德菌(分离 CSRS12)、铜绿假单胞菌(分离 PPRS17)和鲍曼不动杆菌(分离 TA1PS)。B. arboris CSRS12 分离株显示出多种 PGP 特性,如磷酸盐、钾和锌的矿物质溶解、铁载体和氨的产生。在所有三种 PGPR 处理中,B. arboris CSRS12 分离株在盆栽试验中显示出绿豆(Vigna radiata)的侧根数、根和枝条长度、根和枝条鲜重和干重的显着增加。结果表明,CSRS12 分离株可用作生物接种剂,可促进驯化作物和野生品种提高生产力和生态动态。arboris CSRS12 分离株显示出多种 PGP 特性,如磷酸盐、钾和锌的矿物质溶解、铁载体和氨的产生。在所有三种 PGPR 处理中,B. arboris CSRS12 分离株在盆栽试验中显示出绿豆(Vigna radiata)的侧根数、根和枝条长度、根和枝条鲜重和干重的显着增加。结果表明,CSRS12 分离株可用作生物接种剂,可促进驯化作物和野生品种提高生产力和生态动态。arboris CSRS12 分离株显示出多种 PGP 特性,如磷酸盐、钾和锌的矿物质溶解、铁载体和氨的产生。在所有三种 PGPR 处理中,B. arboris CSRS12 分离株在盆栽试验中显示出绿豆(Vigna radiata)的侧根数、根和枝条长度、根和枝条鲜重和干重的显着增加。结果表明,CSRS12 分离株可用作生物接种剂,可促进驯化作物和野生品种提高生产力和生态动态。盆栽试验中绿豆(Vigna radiata)根和芽的鲜重和干重。结果表明,CSRS12 分离株可用作生物接种剂,可促进驯化作物和野生品种提高生产力和生态动态。盆栽试验中绿豆(Vigna radiata)根和芽的鲜重和干重。结果表明,CSRS12 分离株可用作生物接种剂,可促进驯化作物和野生品种提高生产力和生态动态。
更新日期:2020-08-28
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