An in vitro system employing collagen isolated from the sheep tendons to induce mineralization and demineralization reactions was used not only to study the effect of various concentrations of fluoride on the collagen-induced mineralization and demineralization reactions but also to compare their action with the inhibitors of mineralization and/or demineralization. Studies demonstrated that under physiological conditions, at lower concentrations (5 × 10⁻⁶ to 5 × 10⁻⁵ M) fluoride inhibited while at higher concentrations (> 10⁻⁴ M), it stimulated the collagen-induced in vitro mineralization. At higher concentrations, fluoride was also found to inhibit the demineralization of the collagen bound preformed mineral phase. At low concentrations, fluoride acted like Mg²⁺ to inhibit mineralization while at higher concentration, it acted like crystal poisons (e.g., pyrophosphate phosphonates, citrate) to inhibit demineralization. However, unlike magnesium and pyrophosphate, fluoride at its higher concentrations was found to stimulate rather than inhibit the process of mineralization.

使用从绵羊肌腱中分离出的胶原蛋白诱导矿化和脱矿质反应的体外系统，不仅用于研究各种浓度的氟化物对胶原蛋白诱导的矿化和脱矿质反应的影响，而且还比较了它们与矿化抑制剂的作用。和/或脱矿质。研究表明，在生理条件下，较低浓度（5×10 ^-6至5×10 ^-5 M）的氟化物被抑制，而较高浓度（> 10 ^-4）M），它刺激了胶原蛋白诱导的体外矿化作用。在更高的浓度下，还发现氟化物会抑制胶原结合的预形成矿物质相的脱矿质作用。在低浓度下，氟化物像Mg ²⁺一样抑制矿化，而在较高浓度下，它像晶体毒物（例如焦磷酸盐，磷酸盐，柠檬酸盐）一样作用，以抑制脱矿质。但是，与镁和焦磷酸盐不同，发现较高浓度的氟化物会刺激而不是抑制矿化过程。