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Complete genome sequence and genome organization of achyranthes virus A, a novel member of the genus Potyvirus.
Archives of Virology ( IF 2.7 ) Pub Date : 2020-08-26 , DOI: 10.1007/s00705-020-04778-1
Davaajargal Igori 1 , Seungmo Lim 2 , Suk-Yoon Kwon 3 , Hye Sun Cho 3 , Jeong Mee Park 3 , Hyun-Soon Kim 3 , Hyo-Jun Lee 3 , Su-Heon Lee 4 , Jae Sun Moon 3
Affiliation  

The complete genomic sequence of achyranthes virus A (AcVA), from an Achyranthes bidentata Blume plant in South Korea, was determined. The genomic RNA has 9491 nucleotides (nt), excluding the 3′-terminal poly(A) tail and contains an open reading frame typical of members of the genus Potyvirus, family Potyviridae, encoding a large putative polyprotein of 3103 amino acids (aa). Pairwise comparisons showed that the AcVA sequence shares 47.81–57.78% nt sequence identity at the complete genome level, 41.89–56.41% aa sequence identity at the polyprotein level, and 50–63.8% aa sequence identity at the coat protein level with other members of genus Potyvirus. These pairwise comparison values are below the species demarcation cutoff for the family Potyviridae. Our results therefore suggest that this virus should be regarded as a novel member of the genus Potyvirus, tentatively named “achyranthes virus A”.



中文翻译:

牛膝病毒A(波多病毒属的新成员)的完整基因组序列和基因组组织。

确定了来自韩国的牛膝草的牛膝病毒A(AcVA)的完整基因组序列。基因组RNA具有9491个核苷酸(nt),不包括3'-末端poly(A)尾部,并且包含一个典型的开放读框,该开放读框是PotyvirusPotyviridae家族成员的典型密码子,编码一个大型推定的3103个氨基酸的多聚蛋白(aa) 。成对比较显示,AcVA序列与完整的基因组水平在整个基因组水平上具有47.81–57.78%nt序列同一性,在多蛋白水平上具有41.89–56.41%aa序列同一性,在外壳蛋白水平上具有50–63.8%aa序列同一性。属马铃薯Y病毒。这些成对的比较值低于Potyviridae科的物种界限。因此,我们的结果表明,该病毒应被视为马铃薯病毒属的一种新型成员,暂定名为“阿基兰特病毒A”。

更新日期:2020-10-11
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