Retinoblastoma (RB) is an intraocular malignancy that mainly occurs in infants and young children under 5 years of age. Circular RNA hsa_circ_0000034 (circ_0000034) was reported to be upregulated in RB tissues. Nevertheless, the function and mechanism of circ_0000034 in RB are unclear. Expression of circ_0000034, microRNA-361-3p (miR-361-3p), and a disintegrin and metalloproteinase 19 (ADAM19) was examined via quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, migration, invasion, and apoptosis were determined though Cell Counting Kit-8 (CCK-8), transwell, or flow cytometry assays. Caspase-3 activity was detected using a caspase-3 activity assay kit. Some protein levels were examined using Western blot analysis. Dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, or RNA pull-down assay were performed to verify the relationship between circ_0000034 or ADAM19 and miR-361-3p. The function of circ_0000034 in vivo was confirmed via animal experiment. We verified that circ_0000034 expression was elevated in RB tissues and cells. Circ_0000034 silencing reduced RB growth in vivo, repressed viability, migration, invasion, and EMT, and induced apoptosis of RB cells in vitro. Circ_0000034 acted as a sponge for miR-361-3p, which targeted ADAM19 in RB cells. Furthermore, the inhibition of miR-361-3p restored circ_0000034 knockdown-mediated impacts on viability, migration, invasion, apoptosis, and EMT of RB cells. Moreover, ADAM19 overexpression abolished the influence of miR-361-3p mimic on viability, migration, invasion, apoptosis, and EMT of RB cells. Circ_0000034 expedited RB progression through upregulating ADAM19 via sponging miR-361-3p, which indicated that circ_0000034 might a target for RB therapy.

中文翻译：

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环状 RNA has_circ_0000034 通过 miR-361-3p/ADAM19 轴加速视网膜母细胞瘤的进展。

视网膜母细胞瘤（RB）是一种眼内恶性肿瘤，主要发生在5岁以下的婴幼儿。据报道，环状 RNA hsa_circ_0000034 (circ_0000034) 在 RB 组织中上调。然而，circ_0000034在RB中的功能和机制尚不清楚。通过定量实时聚合酶链式反应 (qRT-PCR) 检查 circ_0000034、microRNA-361-3p (miR-361-3p) 和去整合素和金属蛋白酶 19 (ADAM19) 的表达。通过 Cell Counting Kit-8 (CCK-8)、transwell 或流式细胞术测定细胞活力、迁移、侵袭和凋亡。使用 caspase-3 活性测定试剂盒检测 Caspase-3 活性。使用蛋白质印迹分析检查了一些蛋白质水平。双荧光素酶报告基因检测、RNA 免疫沉淀 (RIP) 检测、或 RNA pull-down 测定以验证 circ_0000034 或 ADAM19 与 miR-361-3p 之间的关系。通过动物实验证实了circ_0000034在体内的功能。我们验证了 circ_0000034 表达在 RB 组织和细胞中升高。Circ_0000034 沉默可减少体内 RB 生长，抑制活力、迁移、侵袭和 EMT，并在体外诱导 RB 细胞凋亡。Circ_0000034 充当 miR-361-3p 的海绵，靶向 RB 细胞中的 ADAM19。此外，抑制 miR-361-3p 恢复了 circ_0000034 敲低介导的对 RB 细胞活力、迁移、侵袭、凋亡和 EMT 的影响。此外，ADAM19 过表达消除了 miR-361-3p 模拟物对 RB 细胞活力、迁移、侵袭、凋亡和 EMT 的影响。