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Eosin Y as a high-efficient photooxidase mimic for colorimetric detection of sodium azide.
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2020-08-26 , DOI: 10.1007/s00216-020-02895-z
Junren Wang 1 , Haili Yu 1 , Yi He 1
Affiliation  

The reported fluorescent dye-based artificial light-responsive oxidase mimics are suffering from their low catalytic efficiency. To overcome the limitation, we report the photooxidase-mimicking activity of Eosin Y which can catalyze the oxidation of various chromogenic substrates such as 3,3',5,5'-tetramethylbenzydine (TMB), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 3,3'-diaminobenzidine (DAB), and o-phenylenediamine (OPD) by dissolved oxygen. The photooxidase-like activity of Eosin Y is highly efficient for TMB substrate, and its catalytic efficiency is higher than that of the reported fluorescein (130 fold) and 9-mesityl-10-methylacridinium ion (7.7-fold) mimetic photooxidase. Moreover, the photosensitized Eosin Y-TMB chromogenic system is utilized for colorimetric detection of highly toxic and explosive sodium azide (NaN3) in a linear range from 5 to 500 μM with a limit of detection of 3.5 μM. The resulting colorimetric assay is selective and applied to determine NaN3 in real lake water samples.

中文翻译:

曙红Y是一种高效的光氧化酶模拟物,用于叠氮化钠的比色检测。

报道的基于荧光染料的人造光响应氧化酶模拟物正遭受其低催化效率的困扰。为了克服这一局限性,我们报道了曙红Y的光氧化酶模拟活性,它可以催化各种生色底物,例如3,3',5,5'-四甲基苯并(TMB),2,2'-叠氮基-双( 3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS),3,3'-二氨基联苯胺(DAB)和邻苯二胺(OPD)通过溶解氧产生。曙红Y的类光氧化酶活性对TMB底物非常有效,其催化效率高于报道的荧光素(130倍)和9-间甲-10-甲基ac啶离子(7.7倍)模拟光氧化酶。此外,光敏曙红Y-TMB显色系统用于比色检测高毒性和爆炸性叠氮化钠(NaN3),线性范围为5至500μM,检测极限为3.5μM。所得的比色测定具有选择性,可用于测定真实湖水样品中的NaN3。
更新日期:2020-08-26
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